Mallavia L P, Weiss E
J Bacteriol. 1970 Jan;101(1):127-32. doi: 10.1128/jb.101.1.127-132.1970.
Glutamate was catabolized at a rapid rate by Neisseria meningitidis, group B. Surprisingly, there was a lag of 5 to 30 min in respiration, but not in CO(2) production from C(1), and an appreciable amount of succinate accumulated. The eventual rapid rate of respiration was not prevented by the addition of chloramphenicol. The lag period was eliminated by combinations of substrates that favored the activity of a glutamate-oxaloacetate transaminase. It is suggested that with glutamate as the sole substrate, the reaction terminated at succinate, required only moderate O(2) uptake, and did not result in the transport of succinate to enzymatic sites. The lag period represented the time required for the accumulation of succinate and its transport to enzymatic sites by energy provided by the metabolism of the remaining glutamate. When the transaminase was operative, on the other hand, successive products of the reaction were immediately placed in contact with enzymatic sites.
B群脑膜炎奈瑟菌能快速分解代谢谷氨酸。令人惊讶的是，呼吸作用存在5至30分钟的延迟，但C1的二氧化碳产生却没有延迟，并且积累了相当数量的琥珀酸。添加氯霉素并不能阻止最终快速的呼吸速率。通过有利于谷氨酸-草酰乙酸转氨酶活性的底物组合消除了延迟期。有人提出，以谷氨酸作为唯一底物时，反应终止于琥珀酸，仅需要适度的氧气摄取，并且不会导致琥珀酸转运至酶作用位点。延迟期代表了琥珀酸积累以及通过剩余谷氨酸代谢提供的能量将其转运至酶作用位点所需的时间。另一方面，当转氨酶起作用时，反应的连续产物会立即与酶作用位点接触。
