Jones T R, Muzithras V P, Gluzman Y
Molecular Biology Section, American Cyanamid Co., Pearl River, New York 10965.
J Virol. 1991 Nov;65(11):5860-72. doi: 10.1128/JVI.65.11.5860-5872.1991.
The US6 gene family, located within the unique short region (US) of the human cytomegalovirus (HCMV) genome, contains six open reading frames (US6 through US11) which may encode glycoproteins, such as gcII (D. Gretch, B. Kari, R. Gehrz, and M. Stinski, J. Virol. 62:1956-1962, 1988). By homologous recombination, several different recombinant HCMV were created which contain a marker gene, beta-glucuronidase, inserted within this gene family. It was demonstrated that beta-glucuronidase has utility as a marker gene for the identification of recombinants in this herpesvirus system, without the occurrence of deletions in other regions of the viral genome. DNA and RNA blot analyses attested to the fidelity of the recombination. Immunoprecipitation experiments using monospecific polyclonal antisera indicated that the US10 and/or US11 gene products were not expressed in the recombinants, as predicted. These results, along with single-cycle growth analyses, indicated that the US10 and US11 gene products are nonessential for virus replication and growth in tissue culture. HCMV recombinants expressing beta-glucuronidase seemed to be genetically stable.
US6基因家族位于人巨细胞病毒（HCMV）基因组的独特短区域（US）内，包含六个开放阅读框（从US6到US11），可能编码糖蛋白，如gcII（D. Gretch、B. Kari、R. Gehrz和M. Stinski，《病毒学杂志》62:1956 - 1962，1988年）。通过同源重组，构建了几种不同的重组HCMV，它们在这个基因家族中插入了一个标记基因β - 葡萄糖醛酸酶。结果表明，β - 葡萄糖醛酸酶作为一种标记基因，可用于在这个疱疹病毒系统中鉴定重组体，且病毒基因组的其他区域不会出现缺失。DNA和RNA印迹分析证实了重组的准确性。使用单特异性多克隆抗血清的免疫沉淀实验表明，如预测的那样，重组体中未表达US10和/或US11基因产物。这些结果以及单周期生长分析表明，US10和US11基因产物对于病毒在组织培养中的复制和生长并非必需。表达β - 葡萄糖醛酸酶的HCMV重组体似乎在基因上是稳定的。
