Oram J D, Downing R G, Akrigg A, Dollery A A, Duggleby C J, Wilkinson G W, Greenaway P J
J Gen Virol. 1982 Mar;59(Pt 1):111-29. doi: 10.1099/0022-1317-59-1-111.
Human cytomegalovirus (HCMV) DNA was digested with restriction endonucleases and the fragments characterized with respect to molecular weight and relative mole proportions. The terminal fragments were identified by digesting HCMV DNA with exonucleases before restriction endonuclease treatment and subsequent gel analysis. The HindIII fragments of HCMV DNA were cloned in Escherichia coli and recombinant plasmids were characterized by digestion with restriction endonucleases and by molecular hybridization with HindIII, Bg/II and XbaI fragments of the virus genome. Data from these experiments were used to construct physical maps of HCMV DNA for the HindIII, Bg/II and XbaI restriction endonucleases. The terminal regions of the genome and the region containing fragment HindIII M were shown to be heterogeneous.
用人巨细胞病毒（HCMV）DNA进行限制性核酸内切酶消化，并对片段的分子量和相对摩尔比例进行表征。通过在限制性核酸内切酶处理和随后的凝胶分析之前用核酸外切酶消化HCMV DNA来鉴定末端片段。将HCMV DNA的HindIII片段克隆到大肠杆菌中，通过用限制性核酸内切酶消化以及与病毒基因组的HindIII、Bg/II和XbaI片段进行分子杂交来表征重组质粒。这些实验的数据用于构建针对HindIII、Bg/II和XbaI限制性核酸内切酶的HCMV DNA物理图谱。基因组的末端区域和包含片段HindIII M的区域显示为异质性的。
