Pangborn J, Marr A G, Robrish S A
Electron Microscope Laboratory and Department of Bacteriology, University of California, Davis, California.
J Bacteriol. 1962 Oct;84(4):669-78. doi: 10.1128/jb.84.4.669-678.1962.
Pangborn, J. (University of California, Davis), Allen G. Marr, and S. A. Robrish. Localization of respiratory enzymes in intracytoplasmic membranes of Azotobacter agilis. J. Bacteriol. 84:669-678. 1962.-Thin sections of the cells of Azotobacter agilis which have been disrupted by sonic treatment, by osmotic shock, or by ballistic disintegration reveal a network of internal membranes in the form of vesicles and tubules. The internal membranes are attached to the envelope. Treatment in a Mickle disintegrator of envelopes emptied of cytoplasm by osmotic shock results in the loss of the internal membranes and a concomitant release of reduced diphosphopyridine nucleotide oxidase from the envelopes. Thus, the intracytoplasmic membranes are the probable locus of the respiratory enzymes of the cell. Thin sections of whole cells show tubular intracytoplasmic membranes which are obscured by ribosomes and other dense cytoplasmic constituents.
庞伯恩，J.（加利福尼亚大学戴维斯分校），艾伦·G. 马尔，以及S. A. 罗布里什。活跃固氮菌胞质内膜中呼吸酶的定位。《细菌学杂志》84:669 - 678。1962年。——经超声处理、渗透压休克或撞击破碎而破裂的活跃固氮菌细胞的超薄切片显示，存在呈囊泡和小管形式的内膜网络。内膜附着于细胞膜。对经渗透压休克排空细胞质的细胞膜进行米克尔组织捣碎机处理，会导致内膜丧失，并伴随二磷酸吡啶核苷酸还原氧化酶从细胞膜中释放出来。因此，胞质内膜可能是细胞呼吸酶的所在部位。完整细胞的超薄切片显示出管状的胞质内膜，这些内膜被核糖体和其他密集的细胞质成分所遮盖。
