Hampar B, Copeland M L
National Institute of Dental Research, U.S. Public Health Service, Bethesda, Maryland.
J Bacteriol. 1965 Jul;90(1):205-12. doi: 10.1128/jb.90.1.205-212.1965.
Hampar, Berge (National Institute of Dental Research, Bethesda, Md.), and Mary Lou Copeland. Persistent herpes simplex virus infection in vitro with cycles of cell destruction and regrowth. J. Bacteriol. 90:205-212. 1965.-The susceptibility of two Chinese hamster cell lines to herpes simplex virus (HSV) was studied from the time of their initiation through successive subcultures. The cells' susceptibility to the cytocidal effects of HSV decreased as the number of cell passages increased. During the early cell passages, the decrease in cell susceptibility to HSV was characterized by an increased time after infection for complete cell destruction to occur, with a concomitant increase in the period when virus could be recovered from supernatant fluids. This was followed by a number of cell passages during which persistent HSV infections were established. The persistent infections were characterized by (i) cycles of virus synthesis and cell destruction followed by regrowth of the cells, (ii) initiation and maintenance under conditions optimal for cell growth in the absence of antibody, (iii) the cells' ability to be passaged while still maintaining their cycling patterns, (iv) a relationship between virus synthesis and cell proliferation, and (v) inability of long-term treatment with antibody to "cure" the persistent infections. The unique characteristics of this HSV infection were compared with other persistent in vitro viral infections.
汉帕尔、伯格（美国国立牙科研究所，马里兰州贝塞斯达）和玛丽·卢·科普兰。单纯疱疹病毒在体外持续感染伴细胞破坏与再生周期。《细菌学杂志》90:205 - 212。1965年。——研究了两种中国仓鼠细胞系从起始培养到连续传代过程中对单纯疱疹病毒（HSV）的敏感性。随着细胞传代次数增加，细胞对HSV杀细胞作用的敏感性降低。在细胞传代早期，细胞对HSV敏感性降低的特征是感染后完全细胞破坏发生的时间增加，同时从培养液上清中可回收病毒的时间也增加。随后经过若干细胞传代，建立了HSV持续感染。持续感染的特征为：（i）病毒合成与细胞破坏周期后细胞再生；（ii）在无抗体存在且细胞生长最佳条件下起始并维持；（iii）细胞在传代时仍能保持其循环模式；（iv）病毒合成与细胞增殖之间的关系；（v）用抗体长期处理无法“治愈”持续感染。将这种HSV感染的独特特征与其他体外持续病毒感染进行了比较。
