体外持续性单纯疱疹病毒感染。II. 病毒定位、细胞破坏程度及病毒传播机制
Persistent cyclic herpes simplex virus infection in vitro. II. Localization of virus, degree of cell destruction, and mechanisms of virus transmission.
作者信息
Hampar B
出版信息
J Bacteriol. 1966 May;91(5):1959-64. doi: 10.1128/jb.91.5.1959-1964.1966.
Hampar, Berge (National Institute of Dental Research, Bethesda, Md.). Persistent cyclic herpes simplex virus infection in vitro. II. Localization of virus, degree of cell destruction, and mechanisms of virus transmission. J. Bacteriol. 91:1959-1964. 1966. The localization of virus, degree of cell destruction, and mechanisms of virus transmission in persistent herpes simplex virus-infected cultures were studied. The major fraction of infectious virus was associated with the medium and a minor fraction was associated with the attached cells. Virus in the medium was further separable into a sedimentable (cellular) fraction and a nonsedimentable (extracellular) fraction. The sedimentable fraction was comprised of cellular debris, most of which appeared to contain viral antigen, and intact cells of which less than 10% contained infectious virus. Cell destruction during the cycle involved more than 99.9% of the maximal number of cells present. Infection could be transmitted by extracellular virus, cell-to-cell transfer, and reattachment of infectious cellular material. The results indicated that transmission by reattachment was probably mediated through the cellular debris rather than the intact cells.
汉帕尔、伯格（国立牙科研究所，马里兰州贝塞斯达）。体外单纯疱疹病毒的持续性循环感染。II.病毒的定位、细胞破坏程度及病毒传播机制。《细菌学杂志》91:1959 - 1964。1966年。对单纯疱疹病毒持续性感染培养物中的病毒定位、细胞破坏程度及病毒传播机制进行了研究。大部分感染性病毒与培养基相关，小部分与贴壁细胞相关。培养基中的病毒可进一步分为可沉淀（细胞性）部分和不可沉淀（细胞外）部分。可沉淀部分由细胞碎片组成，其中大部分似乎含有病毒抗原，完整细胞中含有感染性病毒的不到10%。在这个循环过程中，超过99.9%的现存最大细胞数发生了细胞破坏。感染可通过细胞外病毒、细胞间转移以及感染性细胞物质的重新附着进行传播。结果表明，通过重新附着传播可能是通过细胞碎片而非完整细胞介导的。
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