特定酶抑制剂对中国仓鼠卵巢细胞经紫外线照射后的复制、全基因组DNA修复及基因特异性DNA修复的影响。

Effect of specific enzyme inhibitors on replication, total genome DNA repair and on gene-specific DNA repair after UV irradiation in CHO cells.

作者信息

Jones J C, Stevnsner T, Mattern M R, Bohr V A

机构信息

Division of Cancer Treatment, National Cancer Institute, NIH, Bethesda, MD 20892.

出版信息

Mutat Res. 1991 Sep;255(2):155-62. doi: 10.1016/0921-8777(91)90049-u.

DOI:10.1016/0921-8777(91)90049-u

PMID:1656249

Abstract

We have studied the effect of some specific enzyme inhibitors on DNA repair and replication after UV damage in Chinese hamster ovary cells. The DNA repair was studied at the level of the average, overall genome and also in the active dihydrofolate reductase gene. Replication was measured in the overall genome. We tested inhibitors of DNA polymerase alpha and delta (aphidicolin), of poly(ADPr) polymerase (3-aminobenzamide), of ribonucleotide reductase (hydroxyurea), of topoisomerase I (camptothecin), and of topoisomerase II (merbarone, VP-16). In addition, we tested the effect of the potential topoisomerase I activator, beta-lapachone. All of these compounds inhibited genome replication and all topoisomerase inhibitors affected the overall genome repair; beta-lapachone stimulated it. None of these compounds had any effect on the gene-specific repair.

摘要

我们研究了某些特定酶抑制剂对中国仓鼠卵巢细胞紫外线损伤后DNA修复和复制的影响。在平均、整体基因组水平以及活性二氢叶酸还原酶基因中研究了DNA修复。在整体基因组中测量了复制情况。我们测试了DNA聚合酶α和δ的抑制剂（阿非迪霉素）、聚（ADP-核糖）聚合酶的抑制剂（3-氨基苯甲酰胺）、核糖核苷酸还原酶的抑制剂（羟基脲）、拓扑异构酶I的抑制剂（喜树碱）以及拓扑异构酶II的抑制剂（美巴龙、依托泊苷）。此外，我们测试了潜在的拓扑异构酶I激活剂β-拉帕醌的作用。所有这些化合物均抑制基因组复制，所有拓扑异构酶抑制剂均影响整体基因组修复；β-拉帕醌则刺激整体基因组修复。这些化合物均对基因特异性修复无任何影响。