Panelli Simona, Damiani Giuseppe, Espen Luca, Micheli Gioacchino, Sgaramella Vittorio
CERSA/Fondazione Parco Tecnologico Padano, via Einstein 2, Lodi, Italy.
Gene. 2006 May 10;372:1-7. doi: 10.1016/j.gene.2006.01.032. Epub 2006 Mar 27.
The development of methods for the analysis and comparison of the nucleic acids contained in single cells is an ambitious and challenging goal that may provide useful insights in many physiopathological processes. We review here some of the published protocols for the amplification of whole genomes (WGA). We focus on the reaction known as Multiple Displacement Amplification (MDA), which probably represents the most reliable and efficient WGA protocol developed to date. We discuss some recent advances and applications, as well as some modifications to the reaction, which should improve its use and enlarge its range of applicability possibly to degraded genomes, and also to RNA via complementary DNA.
单细胞中所含核酸的分析和比较方法的发展是一个宏伟且具有挑战性的目标，可能会在许多生理病理过程中提供有用的见解。我们在此回顾一些已发表的全基因组扩增（WGA）方案。我们重点关注被称为多重置换扩增（MDA）的反应，它可能是迄今为止开发的最可靠、最有效的WGA方案。我们讨论了一些最新进展和应用，以及对该反应的一些改进，这些改进应能改善其应用并可能扩大其适用于降解基因组以及通过互补DNA适用于RNA的范围。
