Nakhoul Farid, Abassi Zaid, Morgan Michal, Sussan Sharbel, Mirsky Nitza
Department of Nephrology, Rambam Medical Center, Technion, PO Box 9602, Haifa 31096, Israel.
J Am Soc Nephrol. 2006 Apr;17(4 Suppl 2):S127-31. doi: 10.1681/ASN.2005121333.
Diabetic nephropathy is one of the major complications of diabetes. The glucose tolerance factor (GTF) is a dietary agent extracted from several natural sources; the richest among them is brewer's yeast. Extraction and purification of an active and stable GTF preparation from brewer's yeast previously was successful, and a remarkable decrease in plasma glucose and lipids from administration of GTF to animals with type 1 diabetes was demonstrated. The purpose of the present study was to examine whether GTF affects nephropathy in diabetic rats. The average urinary volume and protein excretion throughout the collection period in diabetic rats was 56.95 +/- 2.2 ml/d and 5.42 +/- 0.95 mg/d, respectively. These values were significantly (P < 0.001 versus baseline values) higher compared with healthy controls (average urine volume 15.12 +/- 0.5 ml/d; average protein excretion 0.15 +/- 0.08 mg/d). Treatment with GTF reduced average urine volume and protein excretion to 29.1 +/- 1.94 ml/d (P < 0.01) and 1.55 +/- 1.17 mg/d (P < 0.05), respectively. Kidney weight, which was elevated in diabetic rats, slightly decreased in diabetic animals that were treated with GTF, in association with reduction of lipid peroxidation levels in the renal cortex and the heart. Endothelial nitric oxide immunoreactivity in the renal cortex of both healthy and diabetic rats that were treated with GTF was remarkably lower than that found in renal cortex of untreated diabetic animals. This study demonstrates that yeast-derived material, GTF, can inhibit the development of nephropathy that is induced by diabetes.
糖尿病肾病是糖尿病的主要并发症之一。葡萄糖耐量因子（GTF）是一种从多种天然来源提取的膳食因子；其中最丰富的来源是啤酒酵母。此前已成功从啤酒酵母中提取并纯化出活性和稳定性良好的GTF制剂，并且已证明给1型糖尿病动物施用GTF后，其血糖和血脂显著降低。本研究的目的是检验GTF是否会影响糖尿病大鼠的肾病。在整个收集期内，糖尿病大鼠的平均尿量和蛋白排泄量分别为56.95±2.2 ml/d和5.42±0.95 mg/d。与健康对照组（平均尿量15.12±0.5 ml/d；平均蛋白排泄量0.15±0.08 mg/d）相比，这些值显著更高（与基线值相比，P<0.001）。用GTF治疗后，平均尿量和蛋白排泄量分别降至29.1±1.94 ml/d（P<0.01）和1.55±1.17 mg/d（P<0.05）。糖尿病大鼠升高的肾脏重量，在用GTF治疗的糖尿病动物中略有下降，同时肾皮质和心脏中的脂质过氧化水平降低。用GTF治疗的健康大鼠和糖尿病大鼠肾皮质中的内皮型一氧化氮免疫反应性明显低于未治疗的糖尿病动物肾皮质中的水平。本研究表明，源自酵母的物质GTF可以抑制糖尿病诱导的肾病发展。
