Liu Yong-hou, Xu Chun-hua, Liu Zhi-gang, Liang Jian-guo, Lai Ren
Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2005 Dec 30;23(6):424-7.
To study the bioactive components in ticks which inhibit platelet aggregation, and to understand the molecular mechanism of tick-host interaction.
Sephadex G-50 gel filtration and high performance liquid chromatography (HPLC) were used to purify the platelet aggregation inhibitor from lxodes sinensis. Its molecular weight and purity were checked by matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry. Platelet-rich plasma (PRP) of rabbit was used to examine the function of platelet aggregation inhibitor.
A purified platelet aggregation inhibitor was identified from L. sinensis with a molecular weight of 8 065. It inhibited platelet aggregation induced by ADP with strong potency. The inhibition of platelet aggregation reached over 90% under a concentration of 10 microg/ml.
An inhibitor of platelet aggregation from L. sinensis was identified, which may play an important role for ticks to successfully get blood meal from their hosts.
研究蜱中抑制血小板聚集的生物活性成分，了解蜱与宿主相互作用的分子机制。
采用葡聚糖G - 50凝胶过滤和高效液相色谱（HPLC）从中华硬蜱中纯化血小板聚集抑制剂。通过基质辅助激光解吸电离飞行时间（MALDI - TOF）质谱法检测其分子量和纯度。用兔富血小板血浆（PRP）检测血小板聚集抑制剂的功能。
从中华硬蜱中鉴定出一种纯化的血小板聚集抑制剂，分子量为8065。它对ADP诱导的血小板聚集有强效抑制作用。在浓度为10微克/毫升时，血小板聚集抑制率超过90%。
鉴定出中华硬蜱的一种血小板聚集抑制剂，其可能对蜱成功从宿主获取血餐起重要作用。
