Schmid C, Pan L, Diss T, Isaacson P G
Department of Histopathology, University College & Middlesex School of Medicine, London, United Kingdom.
Am J Pathol. 1991 Oct;139(4):701-7.
Twenty frozen and 55 paraffin sections of lymphnode specimens from 55 patients with pretreatment Hodgkin's disease (nodular sclerosis Hodgkin's disease, n = 45; mixed cellularity Hodgkin's disease, n = 10) were studied by immunohistochemistry and molecular analysis to determine the phenotype of Hodgkin's and Reed-Sternberg cells (HRS). In all cases the HRS cells were CD45-, and CD30+, and in 43/55 (78%) cases they were CD15+. In 48/55 cases (87%) HRS cells were reactive with at least one B-cell marker (CD19, CD20, CD22, CDw75, MB2), 8/55 cases (14.5%) showed reactivity (mainly cytoplasmic) of a subpopulation of HRS cells with the T-cell markers CD3 and beta F1. All cases that expressed T-cell antigens were also reactive with at least one B-cell marker. In frozen sections, a minority of HRS cells in each case studied showed cytoplasmic positivity for bcl-2 protein. Rearrangement of immunoglobulin heavy chain genes was detected in one case and of T-cell receptor beta chain genes in none. The authors were unable to confirm previous reports of bcl-2 gene rearrangement in Hodgkin's disease. The results strongly support a B lymphocytic origin of HRS cells.
对55例初治霍奇金病患者（结节硬化型霍奇金病45例，混合细胞型霍奇金病10例）的淋巴结标本进行了20份冰冻切片和55份石蜡切片研究，通过免疫组织化学和分子分析来确定霍奇金细胞和里德-斯腾伯格细胞（HRS）的表型。在所有病例中，HRS细胞CD45阴性、CD30阳性，43/55（78%）例CD15阳性。48/55例（87%）HRS细胞与至少一种B细胞标志物（CD19、CD20、CD22、CDw75、MB2）反应，8/55例（14.5%）显示HRS细胞亚群与T细胞标志物CD3和βF1有反应（主要为胞质反应）。所有表达T细胞抗原的病例也与至少一种B细胞标志物反应。在冰冻切片中，所研究的每个病例中的少数HRS细胞显示bcl-2蛋白胞质阳性。仅在1例中检测到免疫球蛋白重链基因重排，未检测到T细胞受体β链基因重排。作者无法证实先前关于霍奇金病中bcl-2基因重排的报道。结果有力地支持了HRS细胞起源于B淋巴细胞。
