Greenway Gillian M, Leelasattarathkul Tapparath, Liawruangrath Saisunee, Wheatley R Alan, Youngvises Napaporn
Department of Chemistry, University of Hull, Hull HU6 7RX, UK.
Analyst. 2006 Apr;131(4):501-8. doi: 10.1039/b513735g. Epub 2006 Jan 18.
A novel ultrasonic flow injection chemiluminescence (FI-CL) manifold for determining hydrogen peroxide (H2O2) has been designed and evaluated. Chemiluminescence obtained from the luminol-H2O2-cobalt(II) reaction was enhanced by applying 120 W of ultrasound for a period of 4 s to the reaction coil in the FI-CL system and this enhancement was verified by comparison with an identical manifold without ultrasound. The system was developed for determining ultra-trace levels of H2O2 and a calibration curve was obtained with a linear portion over the range of 10-200 nmol L(-1) H2O2 (correlation coefficient 0.9945). The detection limit (3sigma) and the quantification limit (LOQ) were found to be 1 x 10(-9) and 3.3 x 10(-9) mol L(-1) respectively and the relative standard deviation was 1.37% for 2 x 10(-7) mol L(-1) H2O2 (n = 10). The method was applied to the determination of trace amounts of H2O2 in purified water and natural water samples without any special pre-treatments.
设计并评估了一种用于测定过氧化氢（H₂O₂）的新型超声流动注射化学发光（FI-CL）流路。通过对FI-CL系统中的反应盘管施加120W超声4 s，可增强鲁米诺-H₂O₂-钴（II）反应产生的化学发光。通过与无超声的相同流路进行比较，验证了这种增强效果。该系统用于测定超痕量水平的H₂O₂，获得了校准曲线，其线性范围为10 - 200 nmol L⁻¹ H₂O₂（相关系数0.9945）。检测限（3σ）和定量限（LOQ）分别为1×10⁻⁹和³.³×10⁻⁹ mol L⁻¹，对于2×10⁻⁷ mol L⁻¹ H₂O₂（n = 10），相对标准偏差为1.37%。该方法无需任何特殊预处理即可用于测定纯化水和天然水样中的痕量H₂O₂。
