Zhang Huixin, Du Lin, Wei Zhihao, Wang Xuemei, Sojic Neso, Zhou Xin, Wang Zonghua
College of Materials Science and Engineering, College of Chemistry and Chemical Engineering, Shandong Sino-Japanese Center for Collaborative Research of Carbon Nanomaterials, Instrumental Analysis Center of Qingdao University, Qingdao University, Shandong, 266071, Qingdao, China.
Bordeaux INP, ISM, UMR CNRS 5255, University of Bordeaux, 33607, Pessac, France.
Anal Bioanal Chem. 2022 Dec;414(29-30):8309-8315. doi: 10.1007/s00216-022-04365-0. Epub 2022 Oct 14.
Electrochemiluminescence (ECL) of luminol is a well-established methodology in analytical chemistry and bioimaging. Developing novel strategies to enhance the ECL signal of this model emitter is a challenging but rewarding task. In this work, we introduced the high-intensity focused ultrasound (HIFU), as a pretreatment means and a non-invasive way to trigger and boost the ECL signal with a 40-fold significant enhancement in the luminol-O system without the addition of exogenous co-reactants. The superoxide anion (O) generated in situ by HIFU was the key initiator for boosting the ECL emission as demonstrated in this study for the first time. This promising co-reactant-free strategy could find potential applications for ultrasensitive ECL detection in the analysis of complex biological entities.
鲁米诺的电化学发光（ECL）是分析化学和生物成像中一种成熟的方法。开发新策略来增强这种模型发光体的ECL信号是一项具有挑战性但很有意义的任务。在这项工作中，我们引入了高强度聚焦超声（HIFU），作为一种预处理手段和非侵入性方法，在不添加外源共反应物的情况下，触发并增强鲁米诺-O体系中的ECL信号，实现了40倍的显著增强。本研究首次证明，HIFU原位产生的超氧阴离子（O）是增强ECL发射的关键引发剂。这种有前景的无共反应物策略在复杂生物实体分析的超灵敏ECL检测中可能具有潜在应用。
