Wang Shuo, Quan Ying, Lee Nanju, Kennedy Ivan R
Tianjin Key Laboratory of Food Nutrition and Safety, Faculty of Food Engineering and Biotechnolgy, Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
J Agric Food Chem. 2006 Apr 5;54(7):2491-5. doi: 10.1021/jf0530401.
A rapid enzyme-linked immunosorbent assay (ELISA) test (microwell plate) and a membrane-based colloidal gold immunoassay in flow-through and lateral-flow formats for the rapid detection of fumonisin B1 (FB1) were developed. The rapid microwell assay can be completed within 20 min with the detection limit of 0.5 +/- 0.2 microg/L. Membrane-based colloidal gold immunoassays had a visual detection limit of 1.0 microg/L for FB1 with the detection time of <10 min. Matrix interference was eliminated by 15-fold dilutions of methanol extracts with buffer. These immunoassays can be used as quantitative or qualitative tools for the rapid detection of FB1 residues in 10-20 min on-site.
开发了一种用于快速检测伏马菌素B1（FB1）的快速酶联免疫吸附测定（ELISA）试验（微孔板）以及流通式和侧流式基于膜的胶体金免疫测定法。快速微孔测定可在20分钟内完成，检测限为0.5±0.2微克/升。基于膜的胶体金免疫测定法对FB1的目视检测限为1.0微克/升，检测时间<10分钟。通过用缓冲液将甲醇提取物稀释15倍消除了基质干扰。这些免疫测定法可用作现场在10 - 20分钟内快速检测FB1残留的定量或定性工具。
