Ji Fang, Mokoena Mduduzi P, Zhao Hongyan, Olaniran Ademola O, Shi Jianrong
Key Lab of Food Quality and Safety of Jiangsu Province-State Key Laboratory Breeding/Key Laboratory of Control Technology and Standard for Agro-Product Safety and Quality/Key Laboratory of Agro-Product Safety Risk Evaluation (Nanjing), Ministry of Agriculture, China/ Institute of Food Quality and Safety, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
University of KwaZulu-Natal, Discipline Microbiology, School of Life Sciences, Westville Campus, Private Bag X54001, Durban, South Africa.
PLoS One. 2017 May 10;12(5):e0175282. doi: 10.1371/journal.pone.0175282. eCollection 2017.
A colloidal gold (ICS) test was developed for rapid detection of zearalenone (ZEN) in wheat samples. The mAb against ZEN was prepared in our laboratory and labelled with colloidal gold as a probe for the ICS test. The conditions were optimized and 30 nm colloidal gold nanoparticles were chosen for optimal performance. Millipore 135 was chosen as the NC membrane for its level of sensitivity. The optimum amount of coated antigen ZEN-OVA and anti-ZEN mAb was 0.5 mg/mL and 8 μg/mL, respectively. The ICS test, which has a detection limit of 15 ng/mL for ZEN, could be completed in 5 min. Analysis of ZEN in 202 wheat samples over three consecutive years revealed that data obtained from the ICS test were in a good agreement with LC-MS/MS data. This result demonstrated that the ICS test could be used as a qualitative tool to screen on-site for ZEN.
开发了一种胶体金（ICS）检测方法，用于快速检测小麦样品中的玉米赤霉烯酮（ZEN）。针对ZEN的单克隆抗体在我们实验室制备，并标记胶体金作为ICS检测的探针。对条件进行了优化，选择30nm的胶体金纳米颗粒以实现最佳性能。选择密理博135作为NC膜，因其灵敏度水平。包被抗原ZEN-OVA和抗ZEN单克隆抗体的最佳量分别为0.5mg/mL和8μg/mL。该ICS检测对ZEN的检测限为15ng/mL，可在5分钟内完成。连续三年对202个小麦样品中的ZEN进行分析，结果表明，ICS检测获得的数据与液相色谱-串联质谱（LC-MS/MS）数据高度吻合。这一结果表明，ICS检测可作为一种定性工具用于现场筛选ZEN。
