Jen Hsiu-Ping, Tsai Yuan-Chien, Su Hsiu-Li, Hsieh You-Zung
Department of Applied Chemistry, National Chiao Tung University, Taiwan ROC.
J Chromatogr A. 2006 Apr 14;1111(2):159-65. doi: 10.1016/j.chroma.2005.05.019. Epub 2005 Jun 16.
We have investigated a rapid, simple, and highly efficient on-line preconcentration method using in micellar electrokinetic chromatography (MEKC) for the analysis of abused drugs. Ketamine is an anesthetic that has been abused as a hallucinogen. We applied the sample sweeping technique first to ketamine and its major metabolite, norketamine, and separated the analytes with MEKC. Several of the sweeping MEKC parameters to effect successful separations, such as the concentration of sodium dodecyl sulfate (SDS), the injection time, and the applied voltage were optimized. The improvements in the number of theoretical plates under the different separation conditions are presented clearly in a three-dimensional representation. The limits of detection were 2.8, 3.4, and 3.3 ng/mL for ketamine, norketamine, and ketamine-D(4), respectively. The enrichment factor for each compound was within the range of 540-800. Experimental results are in agreement with those of analysis conducted by gas chromatography/mass spectroscopy (GC/MS). Therefore, we believe that sweeping, combined with MEKC, represents a suitable complementary method to GC/MS for use in clinical and forensic analyses of ketamine and norketamine.
我们研究了一种快速、简单且高效的在线预浓缩方法，该方法用于在胶束电动色谱法（MEKC）中分析滥用药物。氯胺酮是一种已被滥用作致幻剂的麻醉剂。我们首先将样品清扫技术应用于氯胺酮及其主要代谢物去甲氯胺酮，并通过MEKC分离分析物。对几个影响成功分离的清扫MEKC参数进行了优化，如十二烷基硫酸钠（SDS）浓度、进样时间和施加电压。在不同分离条件下理论塔板数的改进以三维表示清晰呈现。氯胺酮、去甲氯胺酮和氯胺酮-D(4)的检测限分别为2.8、3.4和3.3 ng/mL。每种化合物的富集因子在540 - 800范围内。实验结果与气相色谱/质谱联用（GC/MS）分析结果一致。因此，我们认为清扫技术与MEKC相结合，是用于氯胺酮和去甲氯胺酮临床和法医分析的一种适用于GC/MS的互补方法。
