Boska M
Department of Biochemistry, Wright State University, Kettering, OH.
NMR Biomed. 1991 Aug;4(4):173-81. doi: 10.1002/nbm.1940040404.
Net ATP production rates in exercising human muscle were calculated from 31P MRS kinetic data collected with 1-8 s time resolution. During the exercise, the muscle output was measured using a force transducer. Muscle output (force/unit volume) is then divided by the calculated net ATP used to estimate the ATP cost of force production. These measurements have been performed using an exercise protocol of isometric maximum voluntary contraction of the gastrocnemius/soleus muscle group. Results show that by the end of 30 s of exercise, total ATP production and ATP cost of force production had stabilized and remained constant (within the measurement errors) until the end of the 2-min period. The results also demonstrate that either the ATP cost of force production in the human gastrocnemius/soleus is lower in the first second than at any other time in the 2-min window which was investigated, or that the ATP production is being underestimated at the later time points. These data on ATP production rates, and the data relating to ATP cost of force production in muscle will aid in understanding the causes of muscle failure as well as the progression and treatment of muscle disease.
利用以1 - 8秒时间分辨率收集的31P磁共振波谱动力学数据，计算了运动中人体肌肉的净ATP产生率。在运动过程中，使用力传感器测量肌肉输出。然后将肌肉输出（力/单位体积）除以计算出的净ATP消耗量，以估算产生力的ATP成本。这些测量是使用腓肠肌/比目鱼肌组的等长最大自主收缩运动方案进行的。结果表明，到运动30秒结束时，总ATP产生量和产生力的ATP成本已稳定下来，并在2分钟时间段结束前保持恒定（在测量误差范围内）。结果还表明，要么在最初的一秒钟内，人体腓肠肌/比目鱼肌产生力的ATP成本低于在2分钟观察窗口内的任何其他时间，要么在后期时间点ATP产生量被低估了。这些关于ATP产生率的数据，以及与肌肉产生力的ATP成本相关的数据，将有助于理解肌肉疲劳的原因以及肌肉疾病的进展和治疗。
