Ulmann L, Poisson J P, Blond J P, Bézard J
Unité de Recherche de Nutrition cellulaire et Métabolique, Université de Bourgogne, Dijon, France.
Biochim Biophys Acta. 1991 Nov 5;1086(2):230-6. doi: 10.1016/0005-2760(91)90012-7.
The incorporation of [1-14C]linoleic and [1-14C]stearic acid and of their delta 6 and delta 9 desaturation products (gamma-linolenic and oleic acids, respectively) into different classes of lipids was studied in liver microsomes of rats in function of the diet (blackcurrant seed oil diet, containing gamma-linolenic acid, versus control diet) and in function of age (3, 6 and 9 months). After delta 6 desaturation, total radioactivity was distributed between phospholipids, especially phosphatidylcholine, and neutral lipids. The desaturation product, gamma-linolenic acid, was totally recovered in the phospholipid fraction. Blackcurrant seed oil, which decreased the rate of delta 6 desaturation in 6- and 9-month-old rats, also decreased the incorporation of radioactivity in total phospholipids, especially in phosphatidylcholine. At 6 months of age, after delta 9 desaturation, the majority of radioactivity was recovered in neutral lipids principally as oleic acid, the desaturation product. The precursor, stearic acid, was highly incorporated into phospholipids, especially in rats on a diet of blackcurrant seed oil.
研究了在不同饮食（黑加仑籽油饮食，含γ-亚麻酸，与对照饮食）和不同年龄（3、6和9个月）的大鼠肝脏微粒体中，[1-¹⁴C]亚油酸和[1-¹⁴C]硬脂酸及其Δ⁶和Δ⁹去饱和产物（分别为γ-亚麻酸和油酸）掺入不同类脂质的情况。在Δ⁶去饱和后，总放射性分布在磷脂（尤其是磷脂酰胆碱）和中性脂质之间。去饱和产物γ-亚麻酸完全回收在磷脂部分中。黑加仑籽油降低了6个月和9个月大鼠的Δ⁶去饱和速率，也降低了总磷脂尤其是磷脂酰胆碱中放射性的掺入。在6个月大时，经过Δ⁹去饱和后，大部分放射性主要作为去饱和产物油酸回收在中性脂质中。前体硬脂酸高度掺入磷脂中，尤其是在食用黑加仑籽油饮食的大鼠中。
