[生物和非生物诱导子对丹参毛状根培养物中丹参酮产生的协同作用]

[Synergistic effects of biotic and abiotic elicitors on the production of tanshinones in Salvia miltiorrhiza hairy root culture].

作者信息

Yan Qiong, Hu Zong-Ding, Wu Jian-Yong

机构信息

College of Chemical Engineering, Tianjin University, Tianjin 300072, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2006 Feb;31(3):188-91.

PMID:16572993

Abstract

OBJECTIVE

To investigate the synergistic effects of a biotic elicitor yeast extract and different abiotic elicitors (Ag+, Co2+ and alpha-amino isobutyric acid) on the production of tanshinones in Salvia miltiorrhiza hairy root.

METHOD

Different elicitors and their combinations were added to S. miltiorrhiza hairy root culture and the contents of three major tanshinones (crypotanshinone, tanshinone I and tanshinone II A) were analyzed by HPLC.

RESULT

The combinations of yeast extract with different abiotic elicitors had notable synergistic effects on the tanshinone I and tanshinone II A production but not on crypotanshinone. The combination of yeast extract and Ag+ (300 micromol x L(-1)) yielded the highest tanshinone I content, which was nearly 14-fold of the control, and the synergistic elicitation coefficient was 3.0. The combination of yeast extract and Co2+ (100 micromol L(-1)) led to the highest tanshinone IIA content, which was about 14.5-fold of the control, and the synergistic elicitation coefficient was 2.1. Only yeast extract combined with alpha-amino isobutyric acid (200 micromol x L(-1)) increased the crypotanshinone content more effectively than single elicitors. The highest crypotanshinone content was 1.28 mg x g(-1), about 30-fold of the control with a synergistic elicitation coefficient of 1.3.

CONCLUSION

The elicitation by the combination of a biotic elicitor and an abiotic elicitor can generate a synergistic effect, which is more effective than single elicitors to promote secondary metabolite production in plant tissue cultures.

摘要

目的

研究生物激发子酵母提取物与不同非生物激发子（Ag⁺、Co²⁺和α-氨基异丁酸）对丹参毛状根中丹参酮生成的协同作用。

方法

将不同激发子及其组合添加到丹参毛状根培养物中，采用高效液相色谱法分析三种主要丹参酮（隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA）的含量。

结果

酵母提取物与不同非生物激发子的组合对丹参酮Ⅰ和丹参酮ⅡA的生成具有显著的协同作用，但对隐丹参酮无此作用。酵母提取物与Ag⁺（300 μmol·L⁻¹）组合产生的丹参酮Ⅰ含量最高，几乎是对照的14倍，协同激发系数为3.0。酵母提取物与Co²⁺（100 μmol·L⁻¹）组合导致丹参酮ⅡA含量最高，约为对照的14.5倍，协同激发系数为2.1。只有酵母提取物与α-氨基异丁酸（200 μmol·L⁻¹）组合比单一激发子更有效地提高了隐丹参酮含量。最高隐丹参酮含量为1.28 mg·g⁻¹，约为对照的30倍，协同激发系数为1.3。