[In situ extraction effect of macroporous resins on tanshinones in Salvia miltiorrhiza hairy root culture].

OBJECTIVE

To investigate the in situ extraction effect of macroporous resins on tanshinones in Salvia miltiorrhiza hairy root.

METHOD

Macroporous resins were added to S. miltiorrhiza hairy root culture and the contents of three major tanshinones (crypotanshinone, tanshinone I and tanshinone II(A)) in root, medium and resin were analyzed by HPLC.

RESULT

The macroporous resin X - 5 had the best adsorption effect on total tanshinones among three chosen resins (X - 5, AB - 8, XAD - 4) during the S. miltiorrhiza hairy root culture. The adsorption reached equilibrium after four hours with the addition of the resin X -5. The saturation of X - 5 on total tanshinones was 1.2 mg g)-1) resin and the adsorption ratio reached 92.4%.

CONCLUSION

The macroporous resin X -5 could extract tanshinones in situ effectively during S. miltiorrhiza hairy root culture and simplify the further separation process.