Cao Lin
Ningbo Institute for Drug Control, Ningbo 315040, China.
Zhongguo Zhong Yao Za Zhi. 2007 Sep;32(17):1752-4.
To investigate the in situ extraction effect of macroporous resins on tanshinones in Salvia miltiorrhiza hairy root.
Macroporous resins were added to S. miltiorrhiza hairy root culture and the contents of three major tanshinones (crypotanshinone, tanshinone I and tanshinone II(A)) in root, medium and resin were analyzed by HPLC.
The macroporous resin X - 5 had the best adsorption effect on total tanshinones among three chosen resins (X - 5, AB - 8, XAD - 4) during the S. miltiorrhiza hairy root culture. The adsorption reached equilibrium after four hours with the addition of the resin X -5. The saturation of X - 5 on total tanshinones was 1.2 mg g)-1) resin and the adsorption ratio reached 92.4%.
The macroporous resin X -5 could extract tanshinones in situ effectively during S. miltiorrhiza hairy root culture and simplify the further separation process.
研究大孔树脂对丹参毛状根中丹参酮的原位提取效果。
将大孔树脂加入丹参毛状根培养体系中，采用高效液相色谱法分析根、培养基及树脂中三种主要丹参酮（隐丹参酮、丹参酮Ⅰ和丹参酮ⅡA）的含量。
在所选的三种树脂（X-5、AB-8、XAD-4）中，大孔树脂X-5在丹参毛状根培养过程中对总丹参酮的吸附效果最佳。加入树脂X-5后4小时吸附达到平衡。X-5对总丹参酮的饱和吸附量为1.2mg·g-1树脂，吸附率达92.4%。
大孔树脂X-5在丹参毛状根培养过程中能有效原位提取丹参酮，简化后续分离过程。
