Spodniewska A, Zasadowski A
Division of Veterinary and Environmental Toxicology, Department of Pathology and Pharmacology, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 14, 10-719 Olsztyn, Poland.
Pol J Vet Sci. 2006;9(1):23-9.
The aim of this study was to determine the content of vitamin C in the liver of rats exposed to dimethoate or pyrantel embonate as well as co-intoxication with both agents. Investigations were carried out in two stages. At each stage, the rats were divided into three experimental groups (I-III) and a control (C) group. In the first stage, rats from group I were administered pyrantel embonate at a two-week interval at a dose of 1/2 LD50, while the animals from group II received dimethoate for 28 days at a dose of 1/25 LD50, and those from group III - both mentioned compounds in an identical manner as in groups I and II. In the second stage, the rats from group I received pyrantel embonate at a dose of 1/5 LD50 for 3 consecutive days, while the animals from group II received dimethoate at a dose of 1/10 LD50 for 5 consecutive days, and those from III received both compounds, but pyrantel was administered on day 3, 4 and 5 of dimethoate administration. The concentration of vitamin C after pyrantel embonate and dimethoate administration was influenced not only by doses of the compounds used but also by the manner of their application (single or co-administration). Dimethoate delivered at a dose of 1/25 LD50 evoked an increase in vitamin C concentration that was observed to continue up to the 14th day after the exposure, whereas when applied at a dose of 1/10 LD50 it increased the vitamin C level only at the 3rd hour. A considerable decrease in the vitamin C level was reported after pyrantel treatment at a dose of 1/5 LD50. In rats from groups where the compounds were co-administered, increased level of vitamin C was observed at both stages of the experiment only in the first period after intoxication, i.e. up to the 6th hour.
本研究的目的是测定暴露于乐果或噻嘧啶以及两种药物共同中毒的大鼠肝脏中维生素C的含量。研究分两个阶段进行。在每个阶段，将大鼠分为三个实验组（I - III）和一个对照组（C）。在第一阶段，I组大鼠每隔两周以1/2 LD50的剂量给予噻嘧啶，而II组动物以1/25 LD50的剂量接受乐果处理28天，III组动物则以与I组和II组相同的方式同时接受上述两种化合物。在第二阶段，I组大鼠连续3天以1/5 LD50的剂量接受噻嘧啶，II组动物连续5天以1/10 LD50的剂量接受乐果，III组动物同时接受两种化合物，但在乐果给药的第3、4和5天给予噻嘧啶。噻嘧啶和乐果给药后维生素C的浓度不仅受所用化合物剂量的影响，还受其给药方式（单次或联合给药）的影响。以1/25 LD50的剂量给予乐果会引起维生素C浓度升高，这种升高在暴露后持续至第14天，而以1/10 LD50的剂量应用时，仅在第3小时会提高维生素C水平。据报道，以1/5 LD量给予噻嘧啶后，维生素C水平会显著下降。在同时给予两种化合物的组中，仅在中毒后的第一个时期，即直至第6小时，在实验的两个阶段均观察到维生素C水平升高。
