中等密度马基因图谱的构建。

Construction of a medium-density horse gene map.

作者信息

Perrocheau M, Boutreux V, Chadi S, Mata X, Decaunes P, Raudsepp T, Durkin K, Incarnato D, Iannuzzi L, Lear T L, Hirota K, Hasegawa T, Zhu B, de Jong P, Cribiu E P, Chowdhary B P, Guérin G

机构信息

Département de Génétique animale, Laboratoire de Génétique biochimique et de Cytogénétique, Centre de Recherches de Jouy, INRA, 78350, Jouy-en-Josas, France.

出版信息

Anim Genet. 2006 Apr;37(2):145-55. doi: 10.1111/j.1365-2052.2005.01401.x.

DOI:10.1111/j.1365-2052.2005.01401.x

PMID:16573529

Abstract

A medium-density map of the horse genome (Equus caballus) was constructed using genes evenly distributed over the human genome. Three hundred and twenty-three exonic primer pairs were used to screen the INRA and the CHORI-241 equine BAC libraries by polymerase chain reaction and by filter hybridization respectively. Two hundred and thirty-seven BACs containing equine gene orthologues, confirmed by sequencing, were isolated. The BACs were localized to horse chromosomes by fluorescent in situ hybridization (FISH). Overall, 165 genes were assigned to the equine genomic map by radiation hybrid (RH) (using an equine RH(5000) panel) and/or by FISH mapping. A comparison of localizations of 713 genes mapped on the horse genome and on the human genome revealed 59 homologous segments and 131 conserved segments. Two of these homologies (ECA27/HSA8 and ECA12p/HSA11p) had not been previously identified. An enhanced resolution of conserved and rearranged chromosomal segments presented in this study provides clarification of chromosome evolution history.

摘要

利用均匀分布于人类基因组的基因构建了马基因组（马）的中等密度图谱。分别使用323对外显子引物对通过聚合酶链反应和滤膜杂交筛选法国国家农业研究院（INRA）和CHORI-241马细菌人工染色体（BAC）文库。分离出237个经测序确认含有马基因直系同源物的BAC。通过荧光原位杂交（FISH）将这些BAC定位到马染色体上。总体而言，通过辐射杂交（RH）（使用马RH(5000)板）和/或FISH定位将165个基因定位到马基因组图谱上。对713个定位在马基因组和人类基因组上的基因进行定位比较，发现了59个同源片段和131个保守片段。其中两个同源性（ECA27/HSA8和ECA12p/HSA11p）此前未被发现。本研究中呈现的保守和重排染色体片段分辨率的提高，为染色体进化历史提供了清晰的解释。