用于烟草花叶病毒识别的分子印迹聚合物

Molecularly imprinted polymers for tobacco mosaic virus recognition.

作者信息

Bolisay Linden D, Culver James N, Kofinas Peter

机构信息

Department of Chemical and Biomolecular Engineering, University of Maryland, College Park, MD 20742, USA.

出版信息

Biomaterials. 2006 Aug;27(22):4165-8. doi: 10.1016/j.biomaterials.2006.03.018. Epub 2006 Mar 30.

DOI:10.1016/j.biomaterials.2006.03.018

PMID:16574216

Abstract

Molecular imprinted Polymers (MIP) targeted for Tobacco mosaic virus (TMV) have been synthesized. Batch equilibrium studies using imprinted and non-imprinted polymer hydrogels in TMV and TNV solutions were conducted to determine virus-binding capacities. TMV-imprinted hydrogels showed increased binding to TMV (8.8 mg TMV/gpolymer) compared to non-imprinted hydrogels (4.2 mg TMV/gpolymer). Furthermore, TMV-imprinted hydrogels exhibited increased binding to TMV compared to TNV, while non-imprinted hydrogels bound similar amounts of TMV or TNV. This research has demonstrated that molecular imprinting of viruses can be used to selectively induce binding of target viruses based on shape differences of their virions.

摘要

已合成了针对烟草花叶病毒（TMV）的分子印迹聚合物（MIP）。使用TMV和TNV溶液中的印迹和非印迹聚合物水凝胶进行了批量平衡研究，以确定病毒结合能力。与非印迹水凝胶（4.2 mg TMV/g聚合物）相比，TMV印迹水凝胶对TMV的结合能力增强（8.8 mg TMV/g聚合物）。此外，与TNV相比，TMV印迹水凝胶对TMV的结合能力增强，而非印迹水凝胶对TMV或TNV的结合量相似。这项研究表明，病毒的分子印迹可用于基于病毒粒子的形状差异选择性诱导靶病毒的结合。