用高效液相色谱法分析雷氏胡椒（Piper regnellii (Miq.) C. DC.）苍白变种（var. pallescens (C. DC.) Yunck）中的新木脂素类化合物。

Analysis of neolignans compounds of Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) Yunck by HPLC.

作者信息

Felipe Daniele Fernanda, Filho Benedito Prado Dias, Nakamura Celso Vataru, Franco Selma Lucy, Cortez Diógenes Aparício Garcia

机构信息

Programa de Pós-Graduação em Ciências Farmacêuticas, Departamento de Farmácia e Farmacologia, Universidade Estadual deMaringá, Avenida Colombo 5790, Maringá PR 87020-900, Brazil.

出版信息

J Pharm Biomed Anal. 2006 Jun 16;41(4):1371-5. doi: 10.1016/j.jpba.2006.02.029. Epub 2006 Mar 29.

DOI:10.1016/j.jpba.2006.02.029

PMID:16574367

Abstract

A high performance liquid chromatographic (HPLC) method was developed and validated for quantitative determination of neolignans in extracts of Piper regnellii var. pallescens. The analysis were carried out on a Metasil ODS column (150 mm x 4.6 mm, 5 microm) at 30 degrees C, using as mobile phase acetonitrile-water (60:40, v/v) containing 2% acetic acid. The flow rate was 1.0 ml/min and the detection was at 280 nm. The validation using conocarpan as standard demonstrated that the method presents linearity (linear correlation coefficient=0.9991), precision (relative standard deviation <5%) and accuracy (mean recovery=104.55%) in the concentration range 31.25-500 microg/ml. The limit of detection (LOD) was 1.68 microg/ml and the limit of quantitation was 5.60 microg/ml. This method allowed the identification and quantification of conocarpan, eupomatenoid-5 and eupomatenoid-6 in the hydroethanolic extracts obtained from the leaves, stems and roots by maceration process. All the extracts showed the same chromatographic profile, being that the extract of the roots presented the highest concentration of neolignans.

摘要

建立了一种高效液相色谱（HPLC）法，并对其进行了验证，用于定量测定苍白胡椒变种提取物中的新木脂素。分析在Metasil ODS柱（150 mm×4.6 mm，5μm）上于30℃进行，使用含2%乙酸的乙腈-水（60:40，v/v）作为流动相。流速为1.0 ml/min，检测波长为280 nm。以柯诺卡潘为标准品进行的验证表明，该方法在31.25 - 500μg/ml浓度范围内具有线性（线性相关系数 = 0.9991）、精密度（相对标准偏差<5%）和准确度（平均回收率 = 104.55%）。检测限（LOD）为1.68μg/ml，定量限为5.60μg/ml。该方法可对通过浸渍法从叶、茎和根中获得的水乙醇提取物中的柯诺卡潘、隐丹参酮-5和隐丹参酮-6进行鉴定和定量。所有提取物显示出相同的色谱图，其中根提取物中的新木脂素浓度最高。