Kantwerk-Funke G, Burkart V, Kolb H
Diabetes Research Institute, University of Düsseldorf, Germany.
Clin Exp Immunol. 1991 Nov;86(2):266-70. doi: 10.1111/j.1365-2249.1991.tb05808.x.
Multiple low doses of streptozotocin are known to induce immune-mediated insulin deficient diabetes and depression of immune reactivity. We show here that immune depression by streptozotocin is not general but that some parts of the immune system are stimulated. Spleen cells from streptozotocin-treated mice showed enhanced cytotoxicity against syngeneic islet cells and various tumour cells including insulinoma cells. Several cell types served as effector cells, including macrophages, asialo GM1+ and Lyt-2+ lymphocytes. The increased cytotoxic activity towards islet cells was mostly due to macrophages and to non-asialo GM1+ and non-Lyt-2+ lymphocytes. A higher activation state of macrophages in low dose streptozotocin-treated mice was demonstrated by measurements of superoxide anion release. We conclude that multiple low doses of streptozotocin stimulate 'natural cytotoxicity', i.e. the non-MHC restricted cytotoxic activity of macrophages, T cells and natural killer lymphocytes.
已知多次低剂量链脲佐菌素可诱发免疫介导的胰岛素缺乏型糖尿病并导致免疫反应性降低。我们在此表明，链脲佐菌素引起的免疫抑制并非全身性的，而是免疫系统的某些部分受到刺激。经链脲佐菌素处理的小鼠的脾细胞对同基因胰岛细胞和包括胰岛素瘤细胞在内的各种肿瘤细胞表现出增强的细胞毒性。几种细胞类型可作为效应细胞，包括巨噬细胞、去唾液酸GM1+和Lyt-2+淋巴细胞。对胰岛细胞细胞毒性活性的增加主要归因于巨噬细胞以及非去唾液酸GM1+和非Lyt-2+淋巴细胞。通过测量超氧阴离子释放证明，低剂量链脲佐菌素处理的小鼠中巨噬细胞具有更高的激活状态。我们得出结论，多次低剂量链脲佐菌素刺激“自然细胞毒性”，即巨噬细胞、T细胞和自然杀伤淋巴细胞的非主要组织相容性复合体限制的细胞毒性活性。
