Ishibashi T, Kitahara Y, Harada Y, Harada S, Takamoto M, Ishibashi T
Diabetes. 1980 Jul;29(7):516-23. doi: 10.2337/diab.29.7.516.
The immune responsiveness in streptozotocin (SZ)-induced diabetic mice was studied using the immune responses to sheep red blood cells (SRBC) as an indicator system. In SZ-diabetic mice, the weights of such lymphoid organs as the thymus and spleen were significantly decreased with time after SZ administration, whereas the weight of liver was markedly increased. In SZ-diabetic mice, the level of delayed type hypersensitivity (DTH) to SRBC was not lower than that in normal controls in most cases, although the level of DTH was significantly depressed, on occasion, in SZ-diabetic mice. In contrast, antibody-forming activity, measured as the number of plaque-forming cells (PFC), was markedly decreased in SZ-diabetic mice. It seems that antibody production is more profoundly depressed than is DTH in SZ-diabetic mice. The transfer of normal thymus and bone marrow cells into SZ-diabetic mice caused only a partial restoration of PFC activity. When normal spleen cells were transferred into diabetic irradiated mice, proliferation of spleen cells and production of splenic PFC was greatly reduced as compared with normal irradiated mice. Treatment with insulin completely reversed such depression in the transfer system. These findings suggest that the chronic insulin-deficient diabetic state caused a depression and delay in the proliferation and differentiation of lymphoid cells.
以对绵羊红细胞（SRBC）的免疫反应作为指标体系，研究了链脲佐菌素（SZ）诱导的糖尿病小鼠的免疫反应性。在SZ诱导的糖尿病小鼠中，注射SZ后，胸腺和脾脏等淋巴器官的重量随时间显著下降，而肝脏重量则明显增加。在SZ诱导的糖尿病小鼠中，大多数情况下对SRBC的迟发型超敏反应（DTH）水平不低于正常对照，尽管有时SZ诱导的糖尿病小鼠的DTH水平会显著降低。相比之下，以空斑形成细胞（PFC）数量衡量的抗体形成活性在SZ诱导的糖尿病小鼠中明显降低。在SZ诱导的糖尿病小鼠中，抗体产生似乎比DTH受到更严重的抑制。将正常胸腺和骨髓细胞转移到SZ诱导的糖尿病小鼠中，仅使PFC活性部分恢复。当将正常脾细胞转移到经照射的糖尿病小鼠中时，与正常经照射小鼠相比，脾细胞增殖和脾PFC产生大大减少。胰岛素治疗完全逆转了转移系统中的这种抑制作用。这些发现表明，慢性胰岛素缺乏的糖尿病状态导致淋巴细胞增殖和分化受到抑制和延迟。
