Gu P
Jinagsu Institute of Cancer Research, Nanjing.
Zhonghua Wai Ke Za Zhi. 1991 Jun;29(6):361-4, 397.
Twenty-two samples of resected primary hepatic carcinoma (PHC) and liver tissues of resective margin were studied by flow cytometric single-variable analysis combined with clinical data. The results showed that the DNA content measured by flow cytometry was a relatively independent factor indicating biological behavior in PHC. The DNA content was increased and the great majority of PHC cells were of aneuploid cells (90.0%). DNA histograms varied and the occurrence rate of multi-aneuploid was 35%. The DNA content distributed widely, indicating that PHC cells were heterogeneous. The occurrence rate of aneuploid was 61.9% in histologically negative hepatic tissues of resective margin, and it was not correlated with categories of hepatic resection. The existence of aneuploid cells in liver tissues of resective margin is thus considered an important factor for the recurrence of PHC. We suggest that surgical treatment is inadequate and adjuvant therapy is required.
采用流式细胞术单变量分析并结合临床资料，对22例原发性肝癌（PHC）切除标本及手术切缘肝组织进行研究。结果显示，流式细胞术检测的DNA含量是提示PHC生物学行为的一个相对独立因素。DNA含量增加，绝大多数PHC细胞为非整倍体细胞（90.0%）。DNA直方图多样，多倍体发生率为35%。DNA含量分布广泛，提示PHC细胞具有异质性。手术切缘肝组织学阴性的肝组织中非整倍体发生率为61.9%，且与肝切除类型无关。因此，手术切缘肝组织中存在非整倍体细胞被认为是PHC复发的重要因素。我们建议手术治疗不充分，需要辅助治疗。
