Pugh B F, Tjian R
Howard Hughes Medical Institute, Department of Molecular and Cell Biology, University of California, Berkeley 94720.
Genes Dev. 1991 Nov;5(11):1935-45. doi: 10.1101/gad.5.11.1935.
In eukaryotes, the TATA box-binding protein (TBP) is responsible for nucleating assembly of the transcription initiation machinery. Here, we report that a TFIID complex containing TBP is essential for transcription even at a promoter that lacks a TATA box. Immunopurification of TFIID reveals that the active species in reconstituting TATA-less transcription is a multisubunit complex consisting of TBP and many TBP-associated factors (TAFs).
在真核生物中，TATA 框结合蛋白（TBP）负责启动转录起始机制的组装。在此，我们报告，即使在缺乏 TATA 框的启动子处，含有 TBP 的 TFIID 复合物对于转录也是必不可少的。对 TFIID 进行免疫纯化发现，在重建无 TATA 框转录过程中的活性成分是一个由 TBP 和许多 TBP 相关因子（TAFs）组成的多亚基复合物。
