Bach M K, Brashler J R, Stout B K, Johnson H G, Sanders M E
Hypersensitivity Diseases and Clinical Pharmacology, Upjohn Company, Kalamazoo, Mich.
Int Arch Allergy Appl Immunol. 1991;94(1-4):167-8. doi: 10.1159/000235352.
We are interested in the physiologic mechanisms of eosinophil activation because of the presumed participation of activated eosinophils in the inflammatory sequelae of asthma. Suspecting that other formed elements of the blood may contribute to such an activation, we examined the capacity of platelet-derived growth factor (PDGF), a product of activated platelets, to activate eosinophils. We found that highly purified monkey and human eosinophils, but not guinea pig eosinophils, were activated by PDGF (superoxide anion production) in a dose-dependent fashion. Moreover, this activation was further dependent on a prior 'priming' of the cells by a brief exposure to subthreshold concentrations of phorbol ester. The response was specific for the BB homodimer of PDGF suggesting it is receptor-dependent.
由于推测活化的嗜酸性粒细胞参与哮喘的炎症后遗症，我们对嗜酸性粒细胞活化的生理机制感兴趣。怀疑血液中的其他有形成分可能促成这种活化，我们检测了活化血小板的产物血小板衍生生长因子（PDGF）活化嗜酸性粒细胞的能力。我们发现，高度纯化的猴和人嗜酸性粒细胞，而非豚鼠嗜酸性粒细胞，可被PDGF以剂量依赖方式激活（产生超氧阴离子）。此外，这种激活进一步依赖于细胞通过短暂暴露于亚阈值浓度佛波酯进行的预先“致敏”。该反应对PDGF的BB同二聚体具有特异性，表明其依赖于受体。
