Walker B A
U.B.C. Pulmonary Research Laboratory, University of British Columbia, Vancouver British Columbia, Canada.
Inflammation. 1996 Feb;20(1):11-21. doi: 10.1007/BF01487741.
Extracellular adenosine has pharmacological activity on a wide variety of cell types and may play an important role as an inflammatory modulator with both pro- and anti-inflammatory activities. These studies examine the effects of adenosine on guinea pig pulmonary eosinophils. Adenosine alone did not directly induce superoxide (O2-) production. Pretreatment with adenosine primed the O2- response of guinea pig pulmonary eosinophils following the addition of 1 or 10 microM platelet-activating factor (PAF). Priming was seen at adenosine concentrations greater than 1 microM and was maximal at 100 microM. At this maximal dose, adenosine priming increased the O2- response to 1 microM and 10 microM PAF by 86% and 51%, respectively. Priming by adenosine was not seen when ionomycin or phorbol myristate acid (PMA) were used as agonists. In fura-2 loaded eosinophils, the addition of 100 microM adenosine resulted in a small but significant rise in intracellular calcium of 54.4 +/- 9.2 nM above baseline. In contrast, similar adenosine concentrations had no effect on cytosolic calcium levels in guinea pig neutrophils. These data demonstrate a pro-inflammatory role for adenosine in elicited guinea pig pulmonary eosinophils.
细胞外腺苷对多种细胞类型具有药理活性，可能作为一种具有促炎和抗炎活性的炎症调节剂发挥重要作用。这些研究考察了腺苷对豚鼠肺嗜酸性粒细胞的影响。单独的腺苷不会直接诱导超氧化物（O2-）的产生。在用1或10微摩尔血小板活化因子（PAF）刺激后，用腺苷预处理可引发豚鼠肺嗜酸性粒细胞的O2-反应。在腺苷浓度大于1微摩尔时可观察到引发作用，在100微摩尔时达到最大。在此最大剂量下，腺苷引发作用分别使对1微摩尔和10微摩尔PAF的O2-反应增加了86%和51%。当使用离子霉素或佛波醇肉豆蔻酸酯（PMA）作为激动剂时，未观察到腺苷引发作用。在负载fura-2的嗜酸性粒细胞中，加入100微摩尔腺苷导致细胞内钙水平比基线水平小幅但显著升高54.4±9.2纳摩尔。相比之下，类似的腺苷浓度对豚鼠中性粒细胞的胞质钙水平没有影响。这些数据证明了腺苷在引发的豚鼠肺嗜酸性粒细胞中具有促炎作用。
