Raymond-Denise A, Guillen N
Unité de Pathogénie Microbienne Moléculaire, U199 Institut National de la Santé et de la Recherche Médicale, Institut Pasteur, Paris, France.
J Bacteriol. 1991 Nov;173(22):7084-91. doi: 10.1128/jb.173.22.7084-7091.1991.
A Bacillus subtilis strain deficient in homologous recombination was isolated from a library of Tn917lac insertion mutants. The interrupted locus consists of an open reading frame encoding a 22,823-dalton polypeptide. Analysis of the deduced amino acid sequence revealed 34% identity and 47.3% similarity with the LexA protein from Escherichia coli. The gene was designated dinR. It is located between the recA and thyA genetic markers, at 162 degrees on the B. subtilis chromosome. The dinR gene was shown to be expressed during the entire B. subtilis cellular cycle with at least a threefold increase when cells develop competence. In addition, the use of a merodiploid strain, in which a copy of the wild-type dinR gene coexists with a dinR-lacZ transcriptional fusion, demonstrated that dinR is an SOS gene and that the SOS-induced expression of dinR occurred only when a wild-type copy of dinR was present. In addition, DinR seems to regulate the expression of dinC, another SOS gene.
从Tn917lac插入突变体文库中分离出一株枯草芽孢杆菌同源重组缺陷菌株。中断的基因座由一个编码22,823道尔顿多肽的开放阅读框组成。对推导的氨基酸序列分析表明，它与大肠杆菌的LexA蛋白有34%的同一性和47.3%的相似性。该基因被命名为dinR。它位于枯草芽孢杆菌染色体上recA和thyA遗传标记之间，在162度处。结果表明，dinR基因在整个枯草芽孢杆菌细胞周期中都有表达，当细胞具备感受态时至少增加三倍。此外，使用一个部分二倍体菌株，其中野生型dinR基因的一个拷贝与dinR-lacZ转录融合共存，结果表明dinR是一个SOS基因，并且只有当dinR的野生型拷贝存在时才会发生SOS诱导的dinR表达。此外，DinR似乎调控另一个SOS基因dinC的表达。
