Laboratoire de Virologie, Institut de Biologie Moléculaire et Cellulaire du Centre National de la Recherche Scientifique, 15, rue Descartes, 67000 Strasbourg, France.
Proc Natl Acad Sci U S A. 1987 Oct;84(19):6750-4. doi: 10.1073/pnas.84.19.6750.
Four endochitinases (poly[1,4-(N-acetyl-beta-D-glucosaminide)] glycanohydrolase, EC 3.2.1.14) have been purified from leaves of Nicotiana tabacum cv. Samsun NN reacting hypersensitively to tobacco mosaic virus. Two of them are acidic proteins of molecular weights 27,500 and 28,500 and have been identified as 2 of the 10 pathogenesis-related proteins that are known to accumulate in tobacco in response to stress or pathogen attack. These two pathogenesis-related proteins, named "P" and "Q" when their biological function was unknown, account for one-third of tobacco mosaic virus-induced chitinase activity of tobacco leaves. They are serologically closely related to the two other chitinases, which can be considered as new basic pathogenesis-related proteins. These two basic chitinases exhibit higher molecular weights (32,000 and 34,000) and higher specific enzyme activity than the two acidic isoforms.
四种内切几丁质酶（多聚[1,4-（N-乙酰-β-D-氨基葡萄糖）]聚糖水解酶，EC 3.2.1.14）已从对烟草花叶病毒反应过敏感性的烟草品种 Samsun NN 的叶片中纯化出来。其中两种是酸性蛋白质，分子量为 27500 和 28500，已被鉴定为已知在烟草中响应应激或病原体攻击而积累的 10 种与发病机制相关的蛋白质中的 2 种。这两种与发病机制相关的蛋白质，在其生物学功能未知时被命名为“P”和“Q”，占烟草花叶病毒诱导的烟草叶片几丁质酶活性的三分之一。它们在血清学上与另外两种几丁质酶密切相关，这两种几丁质酶可以被认为是新的基本与发病机制相关的蛋白质。这两种基础几丁质酶表现出比两种酸性同工型更高的分子量（32000 和 34000）和更高的比酶活性。
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