Hooft van Huijsduijnen R A, Cornelissen B J, van Loon L C, van Boom J H, Tromp M, Bol J F
Department of Biochemistry, State University of Leiden, Wassenaarseweg 64, Leiden, The Netherlands.
EMBO J. 1985 Sep;4(9):2167-71. doi: 10.1002/j.1460-2075.1985.tb03911.x.
Infection of Samsun NN tobacco with tobacco mosaic virus (TMV) induces a number of host-encoded, so-called pathogenesis-related (PR-) proteins, which are found in the intercellular space of the leaf and are associated with induced resistance. By immunoprecipitation of their in vitro translation products we were able to detect the mRNAs corresponding to a number of PR-proteins in TMV-infected tobacco, but not in healthy plants. Analysis by the Northern blot technique using cloned cDNA of PR1-mRNAs as probe showed that the mRNAs for the closely related proteins PR1a, 1b and 1c occur at a low level in healthy tobacco; upon TMV infection this level is increased > 100-fold. The PR1-specific probe did no hybridize to mRNAs corresponding to other PR-proteins. Sequencing of the 5'-terminal region of PR1-mRNAs showed that PR1-proteins are derived from precursors by removal of an N-terminal signal peptide of 30 amino acids.
用烟草花叶病毒（TMV）感染萨姆松NN烟草会诱导产生许多宿主编码的所谓病程相关（PR-）蛋白，这些蛋白存在于叶片的细胞间隙中，并与诱导抗性有关。通过对它们的体外翻译产物进行免疫沉淀，我们能够在感染TMV的烟草中检测到与多种PR蛋白相对应的mRNA，但在健康植株中却检测不到。使用PR1-mRNA的克隆cDNA作为探针，通过Northern印迹技术分析表明，密切相关的蛋白PR1a、1b和1c的mRNA在健康烟草中含量很低；TMV感染后，这一水平增加了100倍以上。PR1特异性探针未与对应于其他PR蛋白的mRNA杂交。对PR1-mRNA 5'末端区域进行测序表明，PR1蛋白是通过去除30个氨基酸的N端信号肽从前体衍生而来。
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