Kulakovskaia T V, Trilisenko L V, Lichko L P, Vagabov V M, Kulaev I S
Mikrobiologiia. 2006 Jan-Feb;75(1):35-9.
The inactivation of the PPX1 and PPN1 genes, which encode the major enzymes of polyphosphate degradation (exopolyphosphatase and endopolyphosphatase, respectively), was found to exert different effects on the content of different polyphosphates in the yeast Saccharomyces cerevisiae. The content of relatively low-molecular-weight acid-soluble polyphosphates in mutant yeast strains is inversely proportional to the exopolyphosphatase activity of the cytosol. At the same time, the mutation of these genes exerts no effect on salt-soluble polyphosphates. The content of high-molecular-weight alkali-soluble polyphosphates increases twofold in a mutant with inactivated genes of both exopolyphosphatase and endopolyphosphatase. The data obtained confirm the earlier suggestion that the metabolic pathways of particular polyphosphates in yeasts are different.
编码多聚磷酸盐降解主要酶（分别为胞外多聚磷酸酶和胞内多聚磷酸酶）的PPX1和PPN1基因失活后，对酿酒酵母中不同多聚磷酸盐的含量产生了不同影响。突变酵母菌株中相对低分子量的酸溶性多聚磷酸盐含量与胞质溶胶的胞外多聚磷酸酶活性成反比。同时，这些基因的突变对盐溶性多聚磷酸盐没有影响。在胞外多聚磷酸酶和胞内多聚磷酸酶基因均失活的突变体中，高分子量碱溶性多聚磷酸盐的含量增加了两倍。获得的数据证实了之前的推测，即酵母中特定多聚磷酸盐的代谢途径是不同的。
