Westropp Jodi L, Kass Philip H, Buffington C A T
Department of Veterinary Clinical Sciences, the Ohio State University, Columbus, Ohio 43210, USA.
Am J Vet Res. 2006 Apr;67(4):731-6. doi: 10.2460/ajvr.67.4.731.
To determine the effects of stress in cats with feline idiopathic cystitis (FIC) by evaluating bladder permeability, sympathetic nervous system function, and urine cortisol:creatinine (C:Cr) ratios during periods of stress and after environmental enrichment.
Prospective study.
13 cats with FIC and 12 healthy cats.
Cats subjected to an acute-onset moderate stressor for 8 days received IV injections of fluorescein. Serum fluorescein concentrations were determined and compared with those of controls to evaluate bladder permeability, and urine C:Cr ratios were compared to evaluate function of the hypothalamic-pituitary-adrenal (HPA) axis. Plasma catecholamine concentrations were analyzed in a subset of cats. After 8 days of moderate stress, cats were moved to an enriched environment, and tests were repeated after 21 days.
Serum fluorescein concentrations were significantly higher in cats with FIC at all time points. In the cats in which plasma catecholamine concentrations were determined, concentrations of dihydroxyphenylalanine, norepinephrine, and dihyroxyphenylglycol were significantly higher in cats with FIC at all time points, whereas no differences in urine C:Cr ratio between groups were observed.
Cats with FIC appeared to have altered bladder permeability, most notably during the period of initial stress. The increase in plasma dihydroxyphenylalanine concentration suggests that there may be stress-induced increase in the activity of tyrosine hydroxylase, which catalyzes the rate-limiting step in catecholamine synthesis. In contrast, no effects of stress on C:Cr ratios were observed, which suggests there was dissociation between the sympathetic nervous system and HPA-axis responses to stress.
通过评估应激期间和环境富集后猫的膀胱通透性、交感神经系统功能以及尿皮质醇与肌酐（C:Cr）比值，确定应激对患有特发性膀胱炎（FIC）的猫的影响。
前瞻性研究。
13只患有FIC的猫和12只健康猫。
对经历8天急性中度应激源的猫静脉注射荧光素。测定血清荧光素浓度并与对照组进行比较以评估膀胱通透性，比较尿C:Cr比值以评估下丘脑-垂体-肾上腺（HPA）轴的功能。在一部分猫中分析血浆儿茶酚胺浓度。在中度应激8天后，将猫转移到富集环境中，并在21天后重复测试。
在所有时间点，患有FIC的猫的血清荧光素浓度均显著更高。在测定血浆儿茶酚胺浓度的猫中，患有FIC的猫在所有时间点的二羟基苯丙氨酸、去甲肾上腺素和二羟基苯乙二醇浓度均显著更高，而两组之间的尿C:Cr比值未观察到差异。
患有FIC的猫似乎膀胱通透性发生了改变，最明显的是在初始应激期间。血浆二羟基苯丙氨酸浓度的增加表明可能存在应激诱导的酪氨酸羟化酶活性增加，酪氨酸羟化酶催化儿茶酚胺合成中的限速步骤。相比之下，未观察到应激对C:Cr比值的影响，这表明交感神经系统和HPA轴对应激的反应之间存在分离。
