Clinic of Small Animal Medicine, Center of Clinical Veterinary Medicine, LMU Munich, Germany.
Vet J. 2013 Dec;198(3):625-30. doi: 10.1016/j.tvjl.2013.09.015. Epub 2013 Sep 21.
Feline idiopathic cystitis (FIC) is a common lower urinary tract disorder in cats, which often recurs. Published reports document increased urine fibronectin and thioredoxin concentrations in cats with FIC compared with healthy control cats. Therefore, these proteins might be of interest in the pathophysiology of FIC. The purpose of the present study was to evaluate variations in these urine proteins throughout the course of FIC by assessing their concentrations in urine specimens from cats with a history of obstructive FIC. Urine total protein (TP) was measured using the Bradford assay, while urine fibronectin and thioredoxin concentrations were determined by Western blot analysis. Urine TP was significantly higher in cats with obstructive FIC at presentation (day 0) than in healthy control cats (P<0.01). There were significant decreases in urine TP in cats with obstructive FIC after 3 months (P<0.01). Significantly higher urine fibronectin (P<0.01) and thioredoxin (P<0.05) concentrations were demonstrated in cats with FIC at day 0 compared to control cats, but there was no significant change over time (P>0.05). Increased concentrations of these proteins over time might reflect ongoing structural and pathological alterations to functional processes in the urinary bladders of cats with obstructive FIC.
猫特发性膀胱炎（FIC）是一种常见的猫下尿路疾病，常反复发作。已发表的报告显示，与健康对照组相比，患有 FIC 的猫尿液中的纤维连接蛋白和硫氧还蛋白浓度增加。因此，这些蛋白质可能与 FIC 的病理生理学有关。本研究的目的是通过评估有梗阻性 FIC 病史的猫尿液标本中的这些蛋白质浓度，来评估这些尿液蛋白质在 FIC 病程中的变化。使用 Bradford 测定法测定尿液总蛋白（TP），而通过 Western blot 分析测定尿液纤维连接蛋白和硫氧还蛋白浓度。患有梗阻性 FIC 的猫在就诊时（第 0 天）的尿液 TP 明显高于健康对照组（P<0.01）。患有梗阻性 FIC 的猫在 3 个月后尿液 TP 显著下降（P<0.01）。与对照组相比，患有 FIC 的猫在第 0 天的尿液纤维连接蛋白（P<0.01）和硫氧还蛋白（P<0.05）浓度明显升高，但随时间无明显变化（P>0.05）。这些蛋白质浓度随时间的增加可能反映了患有梗阻性 FIC 的猫的膀胱功能过程中的持续结构和病理改变。
