Tsai T H, Chen C F
National Research Institute of Chinese Medicine, Taipei, Taiwan.
J Chromatogr. 1991 Jul 5;567(2):405-14. doi: 10.1016/0378-4347(91)80146-4.
A high-performance liquid chromatographic method for the separation and determination of 18 alpha-glycyrrhetinic acid and 18 beta-glycyrrhetinic acid has been developed. Indomethacin was used as an internal standard. The drugs were separated on a reversed-phase column and detected by UV detection at a wavelength of 254 nm. Methanol-water-perchloric acid-ammonia (80:20:0.4:0.4, v/v) was used as the mobile phase at pH of 7.0-7.5. The detection limit of both compounds was 0.1 microgram/ml in rat plasma. The method was applied to pharmacokinetic studies of glycyrrhetinic acids in rats. The results suggest that the pharmacokinetics appeared to be non-linear in nature.
已开发出一种用于分离和测定18α-甘草次酸和18β-甘草次酸的高效液相色谱法。吲哚美辛用作内标。药物在反相柱上分离，并通过254nm波长的紫外检测进行检测。甲醇-水-高氯酸-氨水（80:20:0.4:0.4，v/v）用作pH为7.0 - 7.5的流动相。两种化合物在大鼠血浆中的检测限均为0.1微克/毫升。该方法应用于大鼠体内甘草次酸的药代动力学研究。结果表明，药代动力学在本质上似乎是非线性的。
