高效液相色谱法测定人血浆中甘草次酸的含量

Determination of glycyrrhetinic acid in human plasma by high-performance liquid chromatography.

作者信息

Brown-Thomas J M, Christensen R G, Rieger R, Malone W, May W E

机构信息

Organic Analytical Research Division, National Institute of Standards and Technology, Gaithersburg, MD 20899.

出版信息

J Chromatogr. 1991 Jul 17;568(1):232-8. doi: 10.1016/0378-4347(91)80358-j.

DOI:10.1016/0378-4347(91)80358-j

PMID:1770101

Abstract

A high-performance liquid chromatographic (HPLC) method has been developed for measuring 18 beta-glycyrrhetinic acid (GRA) in human plasma in the range of 0.1-3 micrograms/ml. The acetate ester of GRA is added to the plasma as an internal standard, plasma proteins are denatured with urea to release GRA, and the GRA and the internal standard are extracted in an ion-pairing solid-phase extraction process. An isocratic, reversed-phase HPLC separation is used, followed by ultraviolet absorbance detection at 248 nm. The results from the analysis of five GRA-fortified plasma pools show a mean relative standard deviation of 7% and are accurate to within 10%. With evaporative concentration of the extract, the limit of detection for GRA in plasma is approximately 10 ng/ml.

摘要

已开发出一种高效液相色谱（HPLC）方法，用于测定人血浆中浓度范围为0.1 - 3微克/毫升的18β - 甘草次酸（GRA）。将GRA的乙酸酯作为内标加入血浆中，用尿素使血浆蛋白变性以释放GRA，然后通过离子对固相萃取法提取GRA和内标。采用等度反相HPLC分离，随后在248nm处进行紫外吸光度检测。对五个添加了GRA的血浆样本池的分析结果显示，平均相对标准偏差为7%，准确度在10%以内。通过对提取物进行蒸发浓缩，血浆中GRA的检测限约为10纳克/毫升。