McClintock J T, Chan I J, Taub F E, Friedman-Kien A E, Resnick L
Digene Diagnostics, Inc., Silver Spring, Maryland.
J Virol Methods. 1991 Jun;33(1-2):155-64. doi: 10.1016/0166-0934(91)90016-s.
An in situ hybridization technique, using horseradish peroxidase (HRP)-labeled DNA probes containing a portion of the Epstein-Barr virus (EBV) genome, was used to detect EBV DNA in tongue sections and smears from patients with oral lesions resembling the clinical features of oral hairy leukoplakia (HL). Eleven biopsy specimens (six consistent with HL, four normal tongue controls, and one leukoplakia) and 11 tongue smears were evaluated for the presence of EBV, cytomegalovirus (CMV), herpes simplex virus (HSV), and human papillomavirus (HPV) type 16. Following hybridization, six biopsy specimens and 10 tongue smears were found positive for EBV. All biopsy cases were negative for CMV, HSV, HPV-16 and the negative control probe. The specificity of the in situ hybridization assay was 100%. These results suggest that in situ hybridization using HRP-labeled DNA probes may be useful as a rapid diagnostic method for the detection of EBV in tongue sections or smears from patients diagnosed with HL.
采用一种原位杂交技术，使用含有部分爱泼斯坦 - 巴尔病毒（EBV）基因组的辣根过氧化物酶（HRP）标记的DNA探针，来检测口腔病变患者舌组织切片和涂片样本中的EBV DNA，这些患者的口腔病变具有类似口腔毛状白斑（HL）的临床特征。对11份活检标本（6份符合HL，4份正常舌对照，1份白斑）和11份舌涂片进行评估，检测EBV、巨细胞病毒（CMV）、单纯疱疹病毒（HSV）和16型人乳头瘤病毒（HPV）的存在情况。杂交后，发现6份活检标本和10份舌涂片EBV呈阳性。所有活检病例的CMV、HSV、HPV - 16及阴性对照探针检测均为阴性。原位杂交检测的特异性为100%。这些结果表明，使用HRP标记的DNA探针进行原位杂交，可能作为一种快速诊断方法，用于检测诊断为HL患者的舌组织切片或涂片样本中的EBV。
