Cubie H A, Felix D H, Southam J C, Wray D
Regional Virus Laboratory, City Hospital, Edinburgh, Scotland.
J Oral Pathol Med. 1991 Jul;20(6):271-4. doi: 10.1111/j.1600-0714.1991.tb00926.x.
A rapid method for the detection of EBV-DNA in paraffin sections of lesions of oral hairy leukoplakia (OHL) is described. The method makes use of advances in molecular technology, including the use of synthetic oligonucleotides with digoxigenin labelling in an in situ hybridisation (ISH) reaction, which can be completed in 24 h. Using this method, sections from 15 of 17 patients clinically diagnosed as having OHL contained readily detectable EBV-DNA in small foci along the upper layers of the stratum spinosum. The sections examined from the two remaining patients appeared to be EBV-DNA negative but both patients were on AZT therapy and one was in addition receiving acyclovir.
本文描述了一种快速检测口腔毛状白斑（OHL）病变石蜡切片中EBV-DNA的方法。该方法利用了分子技术的进展，包括在原位杂交（ISH）反应中使用地高辛标记的合成寡核苷酸，整个反应可在24小时内完成。使用该方法，在临床诊断为OHL的17例患者中，有15例患者的切片在棘层上层的小病灶中可轻松检测到EBV-DNA。其余两名患者的切片似乎为EBV-DNA阴性，但这两名患者均接受齐多夫定治疗，其中一名还同时接受阿昔洛韦治疗。
