Lee Soo-Youn, Oh Hyeon Ju, Kim Jong Won, Kim Yoon Gyoon, Moon Chul Jin, Lee Eun Hee
Department of Laboratory Medicine, Samsung Medical Center, Sungkyunkwan University School of Medicine, Seoul, Republic of Korea.
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jul 24;839(1-2):118-23. doi: 10.1016/j.jchromb.2006.02.056. Epub 2006 Apr 3.
We developed and validated a simple, rapid, and accurate HPLC-MS/MS method with simple protein precipitation for the determination of orphenadrine. Injection-to-injection running time was 3 min with a retention time of orphenadrine of 1.1 min. The linear assay range was 1-200 ng/mL (r2 > 0.99). The intra- and inter-assay imprecisions were CV 0.6-4.2% and CV 1.6-6.1%, respectively. The accuracy, extraction recovery, specificity and stability were satisfactory. Using the measured plasma concentrations of orphenadrine in 24 healthy subjects, pharmacokinetic profiles of orphenadrine were evaluated (AUC(0-72,) 1565+/-731 ng h/mL, Cmax 82.8+/-26.2 ng/mL, Tmax 3.0+/-0.9 h, elimination half-life 25.8+/-10.3 h).
我们开发并验证了一种简单、快速且准确的HPLC-MS/MS方法，该方法采用简单的蛋白质沉淀法来测定奥芬那君。进样间运行时间为3分钟，奥芬那君的保留时间为1.1分钟。线性测定范围为1-200 ng/mL（r2>0.99）。批内和批间精密度的变异系数分别为CV 0.6-4.2%和CV 1.6-6.1%。准确度、提取回收率、特异性和稳定性均令人满意。利用24名健康受试者中测得的奥芬那君血浆浓度，评估了奥芬那君的药代动力学特征（AUC(0-72,) 1565±731 ng h/mL，Cmax 82.8±26.2 ng/mL，Tmax 3.0±0.9小时，消除半衰期25.8±10.3小时）。
