Walash Mohamed I, Belal Fathalla F, Eid Manal I, Mohamed Samah Abo El Abass
Department of Analytical Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura, 35516, Egypt.
Chem Cent J. 2011 Oct 8;5:60. doi: 10.1186/1752-153X-5-60.
A simple, sensitive and rapid spectrophotometric method was developed and validated for the determination of two skeletal muscle relaxants namely, tizanidine hydrochloride (I) and orphenadrine citrate (II) in pharmaceutical formulations. The proposed method is based on the formation of a binary complex between the studied drugs and eosin Y in aqueous buffered medium (pH 3.5). Under the optimum conditions, the binary complex showed absorption maxima at 545 nm for tizanidine and 542 nm for orphenadrine. The calibration plots were rectilinear over concentration range of 0.5-8 μg/mL and 1-12 μg/mL with limits of detection of 0.1 μg/mL and 0.3 μg/mL for tizanidine and orphenadrine respectively. The different experimental parameters affecting the development and stability of the complex were studied and optimized. The method was successfully applied for determination of the studied drugs in their dosage forms; and to the content uniformity test of tizanidine in tablets.
开发并验证了一种简单、灵敏且快速的分光光度法，用于测定药物制剂中的两种骨骼肌松弛剂，即盐酸替扎尼定（I）和枸橼酸奥芬那君（II）。所提出的方法基于在水性缓冲介质（pH 3.5）中，所研究的药物与曙红Y形成二元络合物。在最佳条件下，二元络合物在545 nm处呈现替扎尼定的吸收最大值，在542 nm处呈现奥芬那君的吸收最大值。校准曲线在0.5 - 8 μg/mL和1 - 12 μg/mL的浓度范围内呈线性，替扎尼定和奥芬那君的检测限分别为0.1 μg/mL和0.3 μg/mL。研究并优化了影响络合物形成和稳定性的不同实验参数。该方法成功应用于测定所研究药物的剂型；并用于片剂中替扎尼定的含量均匀度测试。
