Bandstra Sharon R, Fried Bernard, Sherma Joseph
Department of Chemistry, Lafayette College, Easton, PA 18042, USA.
Parasitol Res. 2006 Sep;99(4):414-8. doi: 10.1007/s00436-006-0180-5. Epub 2006 Apr 1.
This study examined the effects of larval trematode infection on the neutral lipid and phospholipid content of Biomphalaria glabrata patently infected with the daughter rediae of Echinostoma caproni. Uninfected snails were used as matched controls. As determined by qualitative high-performance silica gel thin-layer chromatography (HPTLC), the major neutral lipids present in the whole bodies and digestive gland-gonad complexes in both snail populations were free sterols, free fatty acids, and triacylglycerols, and the major polar lipids were phosphatidylcholine and phosphatidylethanolamine. Quantitative analysis by HPTLC with visible and UV scanning reflectance densitometry showed no significant differences in the concentrations of these lipids in whole bodies of infected snails vs the controls, but the concentration of triacylglycerols in the infected digestive gland-gonad complex was significantly less than that of the uninfected. No qualitative differences in neutral lipids and phospholipids in shell or plasma samples were found between infected vs uninfected snails.
本研究考察了感染卡氏棘口吸虫子雷蚴的光滑双脐螺体内幼虫期吸虫感染对中性脂质和磷脂含量的影响。未感染的螺作为匹配对照。通过定性高效硅胶薄层层析（HPTLC）测定，两个螺种群的整个身体以及消化腺 - 性腺复合体中存在的主要中性脂质为游离固醇、游离脂肪酸和三酰甘油，主要极性脂质为磷脂酰胆碱和磷脂酰乙醇胺。采用可见和紫外扫描反射光密度计的HPTLC定量分析表明，感染螺的整个身体中这些脂质的浓度与对照相比无显著差异，但感染螺的消化腺 - 性腺复合体中三酰甘油的浓度显著低于未感染的。在感染螺和未感染螺之间，壳或血浆样本中的中性脂质和磷脂没有定性差异。
