Mahmoud Momeana B, El-Sayed Kamelia, El-Din Ahmed T Sharaf
Department of Medical Malacology, Theodor Bilharz Research Institute, Imbaba 30, Giza, Egypt.
J Egypt Soc Parasitol. 2013 Aug;43(2):517-26. doi: 10.12816/0006408.
The study examined the effects of larval trematode infection on the neutral lipid and phospholipid content of Biomphalaria alexandrina infected with Schistosoma mansoni. Uninfected snails were used as matched controls. As determined by qualitative high-performance silica gel thin-layer chromatography (HPTLC), the major neutral lipids present in the whole bodies and digestive gland-gonad complexes in both infected and uninfected snail populations were free sterols, free fatty acids, and triacylglycerols, and the major polar lipids were phosphatidylcho-line and phosphatidyl ethanolamine. Quantitative analysis by HPTLC with visible and UV scanning reflectance densitometry showed the snail's digestive gland lipid level was found to be almost halved in 20 days post infection; a more then 80% reduction being visible after the subsequent 40 and 60 days.
该研究考察了曼氏血吸虫感染对感染曼氏血吸虫的亚历山大双脐螺中性脂质和磷脂含量的影响。未感染的螺作为匹配对照。通过定性高效硅胶薄层层析（HPTLC）测定，感染和未感染螺群体的整个身体以及消化腺-性腺复合体中的主要中性脂质为游离固醇、游离脂肪酸和三酰甘油，主要极性脂质为磷脂酰胆碱和磷脂酰乙醇胺。通过HPTLC结合可见光和紫外扫描反射光密度法进行的定量分析表明，感染后20天螺的消化腺脂质水平几乎减半；在随后的40天和60天后可见超过80%的降低。
