通过随机扩增多态性DNA分析检测到的阿根廷产志贺毒素大肠杆菌血清型内多样性。

Intraserotype diversity among Argentinian verocytotoxigenic Escherichia coli detected by random amplified polymorphic DNA analysis.

作者信息

Krüger Alejandra, Padola Nora L, Parma Alberto E, Lucchesi Paula M A

机构信息

Lab. Inmunoquímica y Biotecnología, Depto. Sanidad Animal y Medicina Preventiva, Fac. Cs. Veterinarias, Universidad Nacional del Centro Pcia. Buenos Aires, Pinto 399 (7000), Tandil, Argentina.

出版信息

J Med Microbiol. 2006 May;55(Pt 5):545-549. doi: 10.1099/jmm.0.46343-0.

DOI:10.1099/jmm.0.46343-0

PMID:16585641

Abstract

Most cases of diarrhoea-associated haemolytic uraemic syndrome (HUS) are caused by verocytotoxin-producing Escherichia coli (VTEC). Argentina has the highest worldwide incidence of HUS, but with a lower incidence of VTEC O157 : H7 serotype than non-Latin American countries. A large number of VTEC serotypes have been isolated from cattle and cattle-derived food products in Argentina. The aim of this work was to study intraserotype genetic diversity among these VTEC strains by random amplification of polymorphic DNA (RAPD). Strains were selected that belonged to the same serotype, but had been isolated from different sources (cattle and meat). Intraserotype genetic diversity was detected among strains belonging to O20 : H19, O113 : H21, O117 : H7, O157 : H7, O171 : H2 and O174 : H21, but only one RAPD profile corresponded to strains belonging to O91 : H21, although these isolates were from different sources.

摘要

大多数腹泻相关溶血尿毒综合征（HUS）病例由产志贺毒素大肠杆菌（VTEC）引起。阿根廷是全球HUS发病率最高的国家，但与非拉丁美洲国家相比，VTEC O157 : H7血清型的发病率较低。在阿根廷，已从牛和牛源性食品中分离出大量VTEC血清型。本研究的目的是通过随机扩增多态性DNA（RAPD）研究这些VTEC菌株的血清型内遗传多样性。选择了属于同一血清型但从不同来源（牛和肉）分离的菌株。在属于O20 : H19、O113 : H21、O117 : H7、O157 : H7、O171 : H2和O174 : H21的菌株中检测到血清型内遗传多样性，但只有一个RAPD图谱与属于O91 : H21的菌株相对应，尽管这些分离株来自不同来源。