Shi Song-Lin, Wang Yong-Ye, Liang Ying, Li Qi-Fu
The Key Laboratory of Chinese Ministry of Education for Cell Biology & Tumor Cell Engineering, School of Life Science, Xiamen University, Xiamen 361005, Fujian Province, China.
World J Gastroenterol. 2006 Mar 21;12(11):1694-8. doi: 10.3748/wjg.v12.i11.1694.
To investigate the effects of tachyplesin and n-sodium butyrate on proliferation and gene expression of human gastric adenocarcinoma cell line BGC-823.
Effects of tachyplesin and n-sodium butyrate on proliferation of BGC-823 cells were determined with trypan blue dye exclusion test and HE staining. Effects of tachyplesin and n-sodium butyrate on cell cycle were detected by flow cytometry. Protein levels of c-erbB-2, c-myc, p53 and p16 were examined by immunocytochemistry.
The inhibiting effects were similar after 2.0 mg/L tachyplesin and 2.0 mmol/L n-sodium butyrate treatment, the inhibitory rate of cellular growth was 62.66% and 60.19% respectively, and the respective maximum mitotic index was decreased by 49.35% and 51.69% respectively. Tachyplesin and n-sodium butyrate treatment could markedly increase the proportion of cells at G(0)/G(1) phase and decrease the proportion at S phase. The expression levels of oncogene c-erbB-2, c-myc, and mtp53 proteins were down-regulated while the expression level of tumor suppressor gene p16 protein was up-regulated after the treatment with tachyplesin or n-sodium butyrate. The effects of 1.0 mg/L tachyplesin in combination with 1.0 mmol/L n-sodium butyrate were obviously superior to their individual treatment in changing cell cycle distribution and expression of c-erbB-2, c-myc, mtp53 and p16 protein. The inhibitory rate of cellular growth of BGC-823 cells after combination treatment was 62.29% and the maximum mitotic index was decreased by 51.95%.
Tachyplesin as a differentiation inducer of tumor cells has similar effects as n-sodium butyrate on proliferation of tumor cells, expression of correlative oncogene and tumor suppressor gene. It also has a synergistic effect on differentiation of tumor cells.
探讨鲎素和丁酸钠对人胃腺癌细胞系BGC - 823增殖及基因表达的影响。
采用台盼蓝拒染试验和苏木精 - 伊红染色检测鲎素和丁酸钠对BGC - 823细胞增殖的影响。通过流式细胞术检测鲎素和丁酸钠对细胞周期的影响。采用免疫细胞化学法检测c - erbB - 2、c - myc、p53和p16蛋白水平。
2.0 mg/L鲎素和2.0 mmol/L丁酸钠处理后抑制作用相似，细胞生长抑制率分别为62.66%和60.19%，各自的最大有丝分裂指数分别降低49.35%和51.69%。鲎素和丁酸钠处理可显著增加G(0)/G(1)期细胞比例，降低S期细胞比例。鲎素或丁酸钠处理后，癌基因c - erbB - 2、c - myc和突变型p53蛋白表达水平下调，而抑癌基因p16蛋白表达水平上调。1.0 mg/L鲎素与1.0 mmol/L丁酸钠联合作用在改变细胞周期分布及c - erbB - 2、c - myc、突变型p53和p16蛋白表达方面明显优于单独处理。联合处理后BGC - 823细胞的生长抑制率为62.29%，最大有丝分裂指数降低51.95%。
鲎素作为肿瘤细胞分化诱导剂，在肿瘤细胞增殖、相关癌基因及抑癌基因表达方面与丁酸钠有相似作用，且对肿瘤细胞分化有协同作用。
