Yi Shou-Xiang, Yang Ren-Da, Yan Jie, Chang Xiao-Rong, Ling Ya-Ping
Department of Acupuncture and Moxibustion, Hunan College of Traditional Chinese Medicine, 113 Shaoshan Zhonglu, Changsha 41007, Hunan Province, China.
World J Gastroenterol. 2006 Mar 21;12(11):1761-5. doi: 10.3748/wjg.v12.i11.1761.
To discuss the protective effect of electroacupuncture at the Foot-Yangming Meridian on gastric mucosal lesion, somatostatin (SS) and the expression of SS receptor genes (SSR(1)mRNA ) in rabbits with gastric ulcer and to further explore the relative specificity of meridians and viscera at gene expression level.
Forty rabbits were randomly divided into control group (A), gastric ulcer model group (B), Foot-Yangming Meridian group (C), Foot-Shaoyang Meridian group (D) and Foot-Taiyang Meridian group (E). The gastric ulcer model was prepared by infusing alcohol into stomach. Groups C-E were treated with electro-acupuncture at points along the above meridians using meridian stimulating instruments for 7 d respectively. By the end of treatment, the index of gastric ulcer was determined, the amount of epidermal growth factor(EGF) and somatostatin was measured by radioimmunoassay (RIA). SS-R(1)mRNA expression in gastric mucosa was determined by RT-PCR.
The value of EGF in model group was obviously lower (73.6+/-14.8 vs 91.3+/-14.9 pg/mL, P<0.01) than that in control group. The index of gastric ulcer, content of SS and expression of SSR1mRNA in gastric mucosa were significantly higher than those in control group (24.88+/-6.29 vs 8.50+/-2.98 scores, P<0.01; 2978.6+/-587.6 vs 1852.4+/-361.7 mIU/mL, P<0.01; 2.56+/-0.25 vs 1.04+/-0.36, P<0.01). The value of EGF in Foot-Yangming Meridian group was higher than that in model group (92.2+/-6.7 vs 73.6+/-14.8 pg/mL, P<0.01). The index of gastric ulcer, content of SS and expression of SS-R(1)mRNA in gastric mucosa were significantly lower than those in control group (10.88+/-3.23 vs 24.88+/-6.29 scores, P<0.01; 1800.2+/-488 vs 2978.6+/-587.6 mIU/mL, P<0.01; 1.07+/-0.08 vs 2.56+/-0.25 mIU/mL, P<0.01). Compared to the model group, the content of SS and expression of SSR1mRNA in gastric mucosa in Foot-Shaoyang Meridian group decreased (2441.0+/-488.vs 2978.6+/-587.6 mIU/mL, P<0.05;1.73+/-0.16 vs 2.56+/-0.25 mIU/mL, P<0.01). But the above parameters in Foot-Taiyang Meridian group did not improve and were significantly different from those in Foot-Yangming Meridian group (P<0.05).
Electro-acupuncture at Foot-Yangming Meridian can protect gastric mucosa against injury. The mechanism may be related to the regulation of brain-gut peptides and the expression of SSR(1)mRNA.
探讨电针足阳明经对胃溃疡家兔胃黏膜损伤、生长抑素（SS）及生长抑素受体基因（SSR(1)mRNA）表达的保护作用，并从基因表达水平进一步探讨经络与脏腑的相对特异性。
将40只家兔随机分为对照组（A组）、胃溃疡模型组（B组）、足阳明经组（C组）、足少阳经组（D组）和足太阳经组（E组）。采用灌胃乙醇法制备胃溃疡模型。C-E组分别使用经络刺激仪沿上述经络穴位进行电针治疗7天。治疗结束后，测定胃溃疡指数，采用放射免疫分析法（RIA）检测表皮生长因子（EGF）和生长抑素含量。采用逆转录聚合酶链反应（RT-PCR）法测定胃黏膜中SS-R(1)mRNA表达。
模型组EGF值明显低于对照组（73.6±14.8 vs 91.3±14.9 pg/mL，P<0.01）。胃溃疡指数、胃黏膜中SS含量及SSR1mRNA表达均显著高于对照组（24.88±6.29 vs 8.50±2.98分，P<0.01；2978.6±587.6 vs 1852.4±361.7 mIU/mL，P<0.01；2.56±0.25 vs 1.04±0.36，P<0.01）。足阳明经组EGF值高于模型组（92.2±6.7 vs 73.6±14.8 pg/mL，P<0.01）。胃溃疡指数、胃黏膜中SS含量及SS-R(1)mRNA表达均显著低于对照组（10.88±3.23 vs 24.88±6.29分，P<0.01；1800.2±488 vs 2978.6±587.6 mIU/mL，P<0.01；1.07±0.08 vs 2.56±0.25 mIU/mL，P<0.01）。与模型组比较，足少阳经组胃黏膜中SS含量及SSR1mRNA表达降低（2441.0±488 vs 2978.6±587.6 mIU/mL，P<0.05；1.73±0.16 vs 2.56±0.25 mIU/mL，P<0.01）。但足太阳经组上述指标未改善，且与足阳明经组比较差异有统计学意义（P<0.05）。
电针足阳明经可保护胃黏膜免受损伤。其机制可能与调节脑肠肽及SSR(1)mRNA表达有关。
