Liu Bihui, Zhang Jing, Yi Ruokun, Zhou Xianrong, Long Xingyao, Pan Yanni, Zhao Xin
Chongqing Collaborative Innovation Center for Functional Food, Chongqing University of Education, Chongqing 400067, China.
Chongqing Engineering Research Center of Functional Food, Chongqing University of Education, Chongqing 400067, China.
Foods. 2019 Mar 11;8(3):93. doi: 10.3390/foods8030093.
CQPC08 (LF-CQPC08) is a newly discovered strain of bacteria isolated and identified from traditional pickled vegetables in Sichuan, China. We used 4-nitroquinoline 1-oxide to establish an experimental tongue cancer mouse model to evaluate the preventive effect of LF-CQPC08 on tongue cancer in vivo. subsp. , is a common commercial strain and is used as a positive control to compare the effect with LF-CQPC08. The preventive strength and mechanism of LF-CQPC08 on tongue cancer were determined by measuring the biochemical indicators in mouse serum and tissues. Our results showed LF-CQPC08 inhibits the decline of splenic index, thymus index, percentage of phagocytic macrophages, and phagocytic index effectively. LF-CQPC08 also increased levels of mouse serum granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage-CSF (GM-CSF), immunoglobulin (Ig)G, IgM levels of serum interleukin (IL)-4, IL-12, tumor necrosis factor-alpha, and interferon-gamma levels, thereby inhibiting the decline in immunity caused by tongue cancer. It also increased the activity levels of superoxide dismutase and glutathione peroxidase and decreased the levels of malondialdehyde in the tissues of the tongue cancer mouse model, thereby suppressing the oxidative stress damage in the tissue caused by tongue cancer. Through quantitative PCR, LF-CQPC08 upregulated the mRNA expression of nuclear factor-erythroid 2 related factor 2 (Nrf2), heme oxygenase-1 (HO-1), glutathione-S-transferases-π (GST-π), and Bcl-2-associated X protein (Bax), and downregulated the mRNA expression of p53, p63, p73, phosphatase and tensin homolog (PTEN), B-cell lymphoma 2 (Bcl-2) and B-cell lymphoma-extra large (Bcl-xL) in the tongue tissues of the tongue cancer mouse. These results indicated that LF-CQPC08 reduced the influence of tongue cancer on the immune system and oxidative balance and improved the immunity and enhanced antioxidant capacity of the mouse model, thereby preventing tongue cancer. LF-CQPC08 could be used as a microbial resource with a preventive effect on tongue cancer.
CQPC08（LF-CQPC08）是从中国四川传统泡菜中分离鉴定出的一种新发现的细菌菌株。我们使用4-硝基喹啉1-氧化物建立了实验性舌癌小鼠模型，以评估LF-CQPC08在体内对舌癌的预防作用。亚种，是一种常见的商业菌株，用作阳性对照以与LF-CQPC08的效果进行比较。通过测量小鼠血清和组织中的生化指标来确定LF-CQPC08对舌癌的预防强度和机制。我们的结果表明，LF-CQPC08有效抑制脾指数、胸腺指数、吞噬性巨噬细胞百分比和吞噬指数的下降。LF-CQPC08还提高了小鼠血清粒细胞集落刺激因子（G-CSF）、粒细胞-巨噬细胞集落刺激因子（GM-CSF）、免疫球蛋白（Ig）G、血清白细胞介素（IL）-4、IL-12、肿瘤坏死因子-α和干扰素-γ水平，从而抑制舌癌引起的免疫力下降。它还提高了舌癌小鼠模型组织中超氧化物歧化酶和谷胱甘肽过氧化物酶的活性水平，并降低了丙二醛水平，从而抑制舌癌引起的组织氧化应激损伤。通过定量PCR，LF-CQPC08上调了核因子红细胞2相关因子2（Nrf2）、血红素加氧酶-1（HO-1）、谷胱甘肽-S-转移酶-π（GST-π）和Bcl-2相关X蛋白（Bax）的mRNA表达，并下调了舌癌小鼠舌组织中p53、p63、p73、磷酸酶和张力蛋白同源物（PTEN）、B细胞淋巴瘤2（Bcl-2）和B细胞淋巴瘤-特大（Bcl-xL）的mRNA表达。这些结果表明，LF-CQPC08降低了舌癌对免疫系统和氧化平衡的影响，提高了小鼠模型的免疫力并增强了抗氧化能力，从而预防舌癌。LF-CQPC08可作为对舌癌有预防作用的微生物资源。
