Grinvald A, Haas E, Steinberg I Z
The Weizmann Institute of Science, Rehovot, Israel.
Proc Natl Acad Sci U S A. 1972 Aug;69(8):2273-7. doi: 10.1073/pnas.69.8.2273.
When transfer of electronic excitation energy occurs between a donor-acceptor pair by the Förster mechanism, the decay of fluorescence of the donor follows first-order kinetics, with a rate constant that depends on the distance from donor to acceptor. In a system that contains donor-acceptor pairs of different separations, the fluorescence decay of the donors will not be exponential, but will depend on the distribution function of donor-acceptor distances, f(r). Various approaches are outlined for the extraction of information about f(r) from the decay curve of donor fluorescence. Specifically, if a plausible expression with adjustable parameters is assumed for f(r), numerical methods can be used to evaluate the parameters that yield the closest fit between the observed decay curve and that calculated from the assumed f(r). The technique of fluorescence decay may prove to be useful for determination of distribution functions of end-to-end distances of polymers to the edges of which suitable donor-acceptor chromophore pairs have been attached.
当电子激发能通过Förster机制在供体 - 受体对之间发生转移时，供体荧光的衰减遵循一级动力学，其速率常数取决于供体与受体之间的距离。在包含不同间距供体 - 受体对的系统中，供体的荧光衰减将不是指数形式的，而是取决于供体 - 受体距离的分布函数f(r)。概述了从供体荧光衰减曲线中提取有关f(r)信息的各种方法。具体而言，如果为f(r)假设一个带有可调参数的合理表达式，则可以使用数值方法来评估参数，这些参数能使观察到的衰减曲线与根据假设的f(r)计算出的曲线最接近拟合。荧光衰减技术可能被证明可用于确定聚合物端到端距离的分布函数，聚合物边缘已连接了合适的供体 - 受体发色团对。
