Czuper A, Kuśba J, Lakowicz J R
Faculty of Applied Physics and Mathematics, Gdańsk University of Technology, ul. Narutowicza 11/12, 80-952 Gdańsk, Poland.
Department of Biochemistry and Molecular Biology, Center for Fluorescence Spectroscopy, University of Maryland at Baltimore, 725 West Lombard Street, Baltimore, MD 21201, USA.
J Lumin. 2005 Apr;112(1-4):434-438. doi: 10.1016/j.jlumin.2004.09.067. Epub 2004 Oct 18.
We present the theoretical expression describing dependence of the fluorescence intensity decays on the distance distribution ()between energy donors and acceptors in flexible bichromophoric molecules. The expression allows for multiexponential fluorescence decay of the donor- and acceptor-only molecules and takes into account the possibility of incomplete labeling of the molecules by acceptors. It is assumed that the donors and acceptors are static in space and do not move relative to each other during the excited-state lifetime. The potential application of the obtained expression is evaluation of the parameters of the function ().
我们给出了一个理论表达式，该表达式描述了柔性双色分子中能量供体与受体之间的荧光强度衰减对距离分布（）的依赖性。该表达式考虑了仅供体分子和仅受体分子的多指数荧光衰减，并考虑了受体对分子进行不完全标记的可能性。假设供体和受体在空间中是静态的，并且在激发态寿命期间彼此不相对移动。所得表达式的潜在应用是评估函数（）的参数。
